Heart Performance Determination by Visualization in Larval Fishes: Influence of Alternative Models for Heart Shape and Volume Metadata

Title

• Main Title Heart Performance Determination by Visualization in Larval Fishes: Influence of Alternative Models for Heart Shape and Volume

Creator

• Author: Perrichon, Prescilla
  Creator Type: Personal
  Creator Info: University of North Texas
• Author: Grosell, Martin
  Creator Type: Personal
  Creator Info: University of Miami
• Author: Burggren, Warren W.
  Creator Type: Personal
  Creator Info: University of North Texas

Publisher

• Name: Frontiers Research Foundation
  Place of Publication: Lausanne, Switzerland

Date

• Creation: 2017-07-04

Language

• English

Description

• Content Description: This article assesses the validity of three different heart models applied to digital images of complete cardiac cycles in larval mahi-mahi and red drum.
• Physical Description: 10 p.

Subject

• Keyword: mahi-mahi
• Keyword: red drum
• Keyword: larval fish
• Keyword: heart shape modeling
• Keyword: ventricular volume
• Keyword: stroke volume
• Keyword: cardiac output

Source

• Journal: Frontiers in Physiology, 8(464), Frontiers Research Foundation, July 4, 2017, pp. 1-10

Citation

• Publication Title: Frontiers in Physiology
• Volume: 8
• Article Identifier: 464
• Peer Reviewed: True

Collection

• Name: UNT Scholarly Works
  Code: UNTSW

Institution

• Name: UNT College of Science
  Code: UNTCS

Rights

• Rights Access: public
• Rights License: by

Resource Type

• Article

Format

• Text

Identifier

• DOI: 10.3389/fphys.2017.00464
• Archival Resource Key: ark:/67531/metadc987480

Degree

• Academic Department: Biological Sciences
• Academic Department: Biological Sciences

Note

• Display Note: Abstract: Understanding cardiac function in developing larval fishes is crucial for assessing their physiological condition and overall health. Cardiac output measurements in transparent fish larvae and other vertebrates have long been made by analyzing videos of the beating heart, and modeling this structure using a conventional simple prolate spheroid shape model. However, the larval fish heart changes shape during early development and subsequent maturation, but no consideration has been made of the effect of different heart geometries on cardiac output estimation. The present study assessed the validity of three different heartmodels (the “standard” prolate spheroidmodel as well as a cylinder and cone tip + cylinder model) applied to digital images of complete cardiac cycles in larval mahi-mahi and red drum. The inherent error of each model was determined to allow for more precise calculation of stroke volume and cardiac output. The conventional prolate spheroid and cone tip + cylinder models yielded significantly different stroke volume values at 56 hpf in red drum and from 56 to 104 hpf in mahi. End-diastolic and stroke volumes modeled by just a simple cylinder shape were 30–50% higher compared to the conventional prolate spheroid. However, when these values of stroke volume multiplied by heart rate to calculate cardiac output, no significant differences between models emerged because of considerable variability in heart rate. Essentially, the conventional prolate spheroid shape model provides the simplest measurement with lowest variability of stroke volume and cardiac output. However, assessment of heart function—especially if stroke volume is the focus of the study—should consider larval heart shape, with different models being applied on a species-by-species and developmental stage-by-stage basis for best estimation of cardiac output.