Shotgun protein sequencing.

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Description

A novel experimental and computational technique based on multiple enzymatic digestion of a protein or protein mixture that reconstructs protein sequences from sequences of overlapping peptides is described in this SAND report. This approach, analogous to shotgun sequencing of DNA, is to be used to sequence alternative spliced proteins, to identify post-translational modifications, and to sequence genetically engineered proteins.

Physical Description

18 p.

Creation Information

Faulon, Jean-Loup Michel & Heffelfinger, Grant S. June 1, 2009.

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This report is part of the collection entitled: Office of Scientific & Technical Information Technical Reports and was provided by UNT Libraries Government Documents Department to Digital Library, a digital repository hosted by the UNT Libraries. It has been viewed 16 times . More information about this report can be viewed below.

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Description

A novel experimental and computational technique based on multiple enzymatic digestion of a protein or protein mixture that reconstructs protein sequences from sequences of overlapping peptides is described in this SAND report. This approach, analogous to shotgun sequencing of DNA, is to be used to sequence alternative spliced proteins, to identify post-translational modifications, and to sequence genetically engineered proteins.

Physical Description

18 p.

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Identifier

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  • Report No.: SAND2009-3855
  • Grant Number: AC04-94AL85000
  • DOI: 10.2172/959081 | External Link
  • Office of Scientific & Technical Information Report Number: 959081
  • Archival Resource Key: ark:/67531/metadc930298

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Office of Scientific & Technical Information Technical Reports

Reports, articles and other documents harvested from the Office of Scientific and Technical Information.

Office of Scientific and Technical Information (OSTI) is the Department of Energy (DOE) office that collects, preserves, and disseminates DOE-sponsored research and development (R&D) results that are the outcomes of R&D projects or other funded activities at DOE labs and facilities nationwide and grantees at universities and other institutions.

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Creation Date

  • June 1, 2009

Added to The UNT Digital Library

  • Nov. 13, 2016, 7:26 p.m.

Description Last Updated

  • Dec. 1, 2016, 7:58 p.m.

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Faulon, Jean-Loup Michel & Heffelfinger, Grant S. Shotgun protein sequencing., report, June 1, 2009; United States. (digital.library.unt.edu/ark:/67531/metadc930298/: accessed November 21, 2018), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.