Interaction of E-cadherin and PTEN regulates morphogenesis and growth arrest in human mammary epithelial cells

Thumbnail image of item number 1 in: 'Interaction of E-cadherin and PTEN regulates morphogenesis and growth arrest in human mammary epithelial cells'.
Thumbnail image of item number 2 in: 'Interaction of E-cadherin and PTEN regulates morphogenesis and growth arrest in human mammary epithelial cells'.
Thumbnail image of item number 3 in: 'Interaction of E-cadherin and PTEN regulates morphogenesis and growth arrest in human mammary epithelial cells'.
Thumbnail image of item number 4 in: 'Interaction of E-cadherin and PTEN regulates morphogenesis and growth arrest in human mammary epithelial cells'.
Showing 1-4 of 31 pages in this article.

PDF Version Also Available for Download.

Description

PTEN is a dual function phosphatase with tumor suppressor function compromised in a wide spectrum of cancers. Because tissue polarity and architecture are crucial modulators of normal and malignant behavior, we postulated that PTEN may play a role in maintenance of tissue integrity. We used two non-malignant human mammary epithelial cell lines (HMECs) that form polarized, growth-arrested structures (acini) when cultured in 3-dimensional laminin-rich extracellular matrix gels (3D lrECM). As acini begin to form, PTEN accumulates in both the cytoplasm, and at cell-cell contacts where it colocalizes with E-cadherin/{beta}-catenin complex. Reduction of PTEN levels by shRNA in lrECM prevents formation … continued below

Creation Information

Fournier, Marcia V.; Fata, Jimmie E.; Martin, Katherine J.; Yaswen, Paul & Bissell, Mina J. June 3, 2009.

Context

This article is part of the collection entitled: Office of Scientific & Technical Information Technical Reports and was provided by the UNT Libraries Government Documents Department to the UNT Digital Library, a digital repository hosted by the UNT Libraries. It has been viewed 75 times. More information about this article can be viewed below.

Who

People and organizations associated with either the creation of this article or its content.

Authors

Sponsor

Publisher

Provided By

UNT Libraries Government Documents Department

Serving as both a federal and a state depository library, the UNT Libraries Government Documents Department maintains millions of items in a variety of formats. The department is a member of the FDLP Content Partnerships Program and an Affiliated Archive of the National Archives.

About | Browse this Partner

Contact Us

Corrections Questions

What

Descriptive information to help identify this article. Follow the links below to find similar items on the Digital Library.

Description

PTEN is a dual function phosphatase with tumor suppressor function compromised in a wide spectrum of cancers. Because tissue polarity and architecture are crucial modulators of normal and malignant behavior, we postulated that PTEN may play a role in maintenance of tissue integrity. We used two non-malignant human mammary epithelial cell lines (HMECs) that form polarized, growth-arrested structures (acini) when cultured in 3-dimensional laminin-rich extracellular matrix gels (3D lrECM). As acini begin to form, PTEN accumulates in both the cytoplasm, and at cell-cell contacts where it colocalizes with E-cadherin/{beta}-catenin complex. Reduction of PTEN levels by shRNA in lrECM prevents formation of organized breast acini and disrupts growth arrest. Importantly, disruption of acinar polarity and cell-cell contact by E-cadherin function-blocking antibodies reduces endogenous PTEN protein levels and inhibits its accumulation at cell-cell contacts. Conversely, in SKBR3 breast cancer cells lacking endogenous E-cadherin expression, exogenous introduction of E-cadherin gene causes induction of PTEN expression and its accumulation at sites of cell interactions. These studies provide evidence that E-cadherin regulates both the PTEN protein levels and its recruitment to cell-cell junctions in 3D lrECM indicating a dynamic reciprocity between architectural integrity and the levels and localization of PTEN. This interaction thus appears to be a critical integrator of proliferative and morphogenetic signaling in breast epithelial cells.

Source

  • Journal Name: Cancer Research

Language

Item Type

Identifier

Unique identifying numbers for this article in the Digital Library or other systems.

Collections

This article is part of the following collection of related materials.

Office of Scientific & Technical Information Technical Reports

Reports, articles and other documents harvested from the Office of Scientific and Technical Information.

Office of Scientific and Technical Information (OSTI) is the Department of Energy (DOE) office that collects, preserves, and disseminates DOE-sponsored research and development (R&D) results that are the outcomes of R&D projects or other funded activities at DOE labs and facilities nationwide and grantees at universities and other institutions.

About | Browse this Collection

What responsibilities do I have when using this article?

Digital Files

When

Dates and time periods associated with this article.

Creation Date

  • June 3, 2009

Added to The UNT Digital Library

  • Nov. 13, 2016, 7:26 p.m.

Description Last Updated

  • March 19, 2019, 5:48 p.m.

Usage Statistics

When was this article last used?

Yesterday: 0
Past 30 days: 0
Total Uses: 75
More Statistics

Interact With This Article

Here are some suggestions for what to do next.

Start Reading

Thumbnail image of item number 1 in: 'Interaction of E-cadherin and PTEN regulates morphogenesis and growth arrest in human mammary epithelial cells'.
Thumbnail image of item number 2 in: 'Interaction of E-cadherin and PTEN regulates morphogenesis and growth arrest in human mammary epithelial cells'.
Thumbnail image of item number 3 in: 'Interaction of E-cadherin and PTEN regulates morphogenesis and growth arrest in human mammary epithelial cells'.
Thumbnail image of item number 4 in: 'Interaction of E-cadherin and PTEN regulates morphogenesis and growth arrest in human mammary epithelial cells'.

PDF Version Also Available for Download.

Citations, Rights, Re-Use

International Image Interoperability Framework

IIF Logo

We support the IIIF Presentation API

Links for Robots

Helpful links in machine-readable formats.

Archival Resource Key (ARK)

International Image Interoperability Framework (IIIF)

Metadata Formats

Images

URLs

Stats

Fournier, Marcia V.; Fata, Jimmie E.; Martin, Katherine J.; Yaswen, Paul & Bissell, Mina J. Interaction of E-cadherin and PTEN regulates morphogenesis and growth arrest in human mammary epithelial cells, article, June 3, 2009; Berkeley, California. (https://digital.library.unt.edu/ark:/67531/metadc930133/: accessed April 19, 2024), University of North Texas Libraries, UNT Digital Library, https://digital.library.unt.edu; crediting UNT Libraries Government Documents Department.

Back to Top of Screen