Ce-MXRF: the power of separation with bench top element sensitive detection

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Capillary electrophoresis (CE) is a proven separation technique that offers highly efficient separation, rapid analysis, and minute sample consumption. When combined with a element specific detection scheme, it can be used for chemical speciation of biologically and environmentally relevant species such as metal containing proteins. In this study, a new tool was developed for separation and elemental detection. Specifically, a simple CE apparatus was constructed using a thin-walled fused Si capillary and interfaced with a bench top micro x-ray fluorescence (MXRF) system. X-ray excitation and detection of the separated sample volumes was performed using an EDAX Eagle II micro x-ray ... continued below

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19 p.

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Miller, T. C. (Thomasin C.); Joseph, M. R. (Martha R.) & Havrilla, G. J. (George J.) January 1, 2002.

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Description

Capillary electrophoresis (CE) is a proven separation technique that offers highly efficient separation, rapid analysis, and minute sample consumption. When combined with a element specific detection scheme, it can be used for chemical speciation of biologically and environmentally relevant species such as metal containing proteins. In this study, a new tool was developed for separation and elemental detection. Specifically, a simple CE apparatus was constructed using a thin-walled fused Si capillary and interfaced with a bench top micro x-ray fluorescence (MXRF) system. X-ray excitation and detection of the separated sample volumes was performed using an EDAX Eagle II micro x-ray fluorescence system equipped with a Rh target excitation source and a SiLi detector. It was demonstrated that the system could be used for the separation and detection of two metals from one another, specifically Cu{sup 2+} and Co{sup 2+}. Free Co{sup 2+} could also be isolated from Co{sup 2+} bound to cyanocobalamin (Vitamin B-12). Other systems that were explored were the separation of two organics, ferritin from cyanocobalamin as well as the separation of the different Cu and Zn isoforms of metallothinein. CE-MXRF was also used to separate the important serum isoforms of transferrin. Direct comparisons were made between CE-MXRF system and other elemental separation techniques such as CE-PIXE, CE-synchrotron-XRF, and CE-ICPMS.

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19 p.

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  • Submitted to: Denver X-ray Conference, Colorado Springs, July 29-Aug. 2, 2002

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  • Report No.: LA-UR-02-4576
  • Grant Number: none
  • Office of Scientific & Technical Information Report Number: 976233
  • Archival Resource Key: ark:/67531/metadc929743

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Office of Scientific & Technical Information Technical Reports

Reports, articles and other documents harvested from the Office of Scientific and Technical Information.

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  • January 1, 2002

Added to The UNT Digital Library

  • Nov. 13, 2016, 7:26 p.m.

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  • Dec. 9, 2016, 10:56 p.m.

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Miller, T. C. (Thomasin C.); Joseph, M. R. (Martha R.) & Havrilla, G. J. (George J.). Ce-MXRF: the power of separation with bench top element sensitive detection, article, January 1, 2002; United States. (digital.library.unt.edu/ark:/67531/metadc929743/: accessed October 18, 2018), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.