Estimating Contrast Transfer Function and Associated Parameters by Constrained Nonlinear Optimization

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The three-dimensional reconstruction of macromolecules from two-dimensional single-particle electron images requires determination and correction of the contrast transfer function (CTF) and envelope function. A computational algorithm based on constrained non-linear optimization is developed to estimate the essential parameters in the CTF and envelope function model simultaneously and automatically. The application of this estimation method is demonstrated with focal series images of amorphous carbon film as well as images of ice-embedded icosahedral virus particles suspended across holes.

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Yang, Chao; Jiang, Wen; Chen, Dong-Hua; Adiga, Umesh; Ng, Esmond G. & Chiu, Wah July 28, 2008.

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The three-dimensional reconstruction of macromolecules from two-dimensional single-particle electron images requires determination and correction of the contrast transfer function (CTF) and envelope function. A computational algorithm based on constrained non-linear optimization is developed to estimate the essential parameters in the CTF and envelope function model simultaneously and automatically. The application of this estimation method is demonstrated with focal series images of amorphous carbon film as well as images of ice-embedded icosahedral virus particles suspended across holes.

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31

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  • Journal Name: Journal of Microscopy; Journal Volume: 233; Journal Issue: 3; Related Information: Journal Publication Date: 02/27/2009

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  • Report No.: LBNL-2407E
  • Grant Number: DE-AC02-05CH11231
  • Grant Number: P01GM064692
  • Office of Scientific & Technical Information Report Number: 971841
  • Archival Resource Key: ark:/67531/metadc929206

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Office of Scientific & Technical Information Technical Reports

Reports, articles and other documents harvested from the Office of Scientific and Technical Information.

Office of Scientific and Technical Information (OSTI) is the Department of Energy (DOE) office that collects, preserves, and disseminates DOE-sponsored research and development (R&D) results that are the outcomes of R&D projects or other funded activities at DOE labs and facilities nationwide and grantees at universities and other institutions.

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  • July 28, 2008

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  • Nov. 13, 2016, 7:26 p.m.

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  • Nov. 18, 2016, 4:04 p.m.

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Yang, Chao; Jiang, Wen; Chen, Dong-Hua; Adiga, Umesh; Ng, Esmond G. & Chiu, Wah. Estimating Contrast Transfer Function and Associated Parameters by Constrained Nonlinear Optimization, article, July 28, 2008; Berkeley, California. (digital.library.unt.edu/ark:/67531/metadc929206/: accessed April 20, 2018), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.