Enhancing Carbon Fixation by Metabolic Engineering: A Model System of Complex Network Modulation Page: 1 of 12
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"Enhancing Carbon Fixation by Metabolic Engineering:
A Model System of Complex Network Modulation"
Engineering Research Program
Office of Basic Sciences at the Department of Energy
Grant Number: DE-FG02-99ER15015
Dept. of Chemical Engineering
Massachusetts Institute of Technology
April 10th 2008
In the first two years of this research we focused on the development of a DNA
microarrayS for transcriptional studies in the photosynthetic organism
Synechocystis and the elucidation of the metabolic pathway for biopolymer
synthesis in this organism. In addition we also advanced the molecular biological
tools for metabolic engineering of biopolymer synthesis in Synechocystis and
initiated a series of physiological studies for the elucidation of the carbon fixing
pathways and basic central carbon metabolism of these organisms. During the
last two-year period we focused our attention on the continuation and completion
of the last task, namely, the development of tools for basic investigations of the
physiology of these cells through, primarily, the determination of their metabolic
fluxes. The reason for this decision lies in the importance of fluxes as key
indicators of physiology and the high level of information content they carry in
terms of identifying rate limiting steps in a metabolic pathway.
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Stephanopoulos, Dr. Gregory. Enhancing Carbon Fixation by Metabolic Engineering: A Model System of Complex Network Modulation, report, April 10, 2008; United States. (digital.library.unt.edu/ark:/67531/metadc902236/m1/1/: accessed December 11, 2018), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.