Pseudo-stationary separation materials for highly parallel separations. Page: 3 of 34
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SAND 2005-2015
Unlimited Release
Printed May 2005
Pseudo-Stationary Separation Materials
for Highly Parallel Separations
Anup K Singhl, Christopher Palmer2
1Biosystems Research Department
Sandia National Laboratories
MS 9292
Livermore, CA 94551
2 Department of Chemistry
University of Montana
Missoula, MT 59812
Abstract
Goal of this study was to develop and characterize novel polymeric materials as
pseudostationary phases in electrokinetic chromatography. Fundamental studies have
characterized the chromatographic selectivity of the materials as a function of chemical
structure and molecular conformation. The selectivities of the polymers has been studied
extensively, resulting in a large body of fundamental knowledge regarding the
performance and selectivity of polymeric pseudostationary phases. Two polymers have
also been used for amino acid and peptide separations, and with laser induced
fluorescence detection. The polymers performed well for the separation of derivatized
amino acids, and provided some significant differences in selectivity relative to a
commonly used micellar pseudostationary phase. The polymers did not perform well for
peptide separations. The polymers were compatible with laser induced fluorescence
detection, indicating that they should also be compatible with chip-based separations.
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Singh, Anup K. & Palmer, Christopher (University of Montana, Missoula, MT). Pseudo-stationary separation materials for highly parallel separations., report, May 1, 2005; United States. (https://digital.library.unt.edu/ark:/67531/metadc901721/m1/3/: accessed April 25, 2024), University of North Texas Libraries, UNT Digital Library, https://digital.library.unt.edu; crediting UNT Libraries Government Documents Department.