Genetic Stock Identification, Annual Report of Research 1986.

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The results of the first year's investigation of a 5-year plan to demonstrate and develop a coastwide genetic stock identification (GSI) program are presented. The accomplishments under four specific objectives are outlined below: 1. Improved Efficiency through Direct Entry of Electrophoretic Data into the Computer. A program is described that was developed for direct computer entry o f raw data. This program eliminated the need for key- to-tape processing previously required for estimating compositions of mixed fisheries, and thereby permits immediate use of collected data in estimating compositions of stock mixtures. 2. Expand and Strengthen Oregon Coastal and British Columbia ... continued below

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96 pages

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Milner, George B. December 1, 1986.

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Description

The results of the first year's investigation of a 5-year plan to demonstrate and develop a coastwide genetic stock identification (GSI) program are presented. The accomplishments under four specific objectives are outlined below: 1. Improved Efficiency through Direct Entry of Electrophoretic Data into the Computer. A program is described that was developed for direct computer entry o f raw data. This program eliminated the need for key- to-tape processing previously required for estimating compositions of mixed fisheries, and thereby permits immediate use of collected data in estimating compositions of stock mixtures. 2. Expand and Strengthen Oregon Coastal and British Columbia Baseline Data Set. Electrophoretic screening of approximately 105 loci of samples from 22 stocks resulted in complete data sets for 35 polymorphic and 19 monomorphic loci. These new data are part of the baseline information currently used in estimating mixed stock compositions. 3. Conduct a Pilot GSI Study of Mixed Stock Canadian Troll Fisheries off the West Coast of Vancouver Island. A predominance of lower Columbia River (fall run), Canadian, and Puget Sound stocks was observed for both 1984 and 1985 fisheries . Stocks other than Columbia River, Canadian, and Puget Sound contributed an estimated 13 and 5 % respectively, to the 1984 and 1985 fisheries . 4. Validation of GSI for Estimating Mixed Fishery Stock Composition. Baseline data from the Columbia River southward were used to simulate nor them and central California fisheries . These simulations provided estimates of accuracy and precision for mixed sample sizes ranging from 250 to 1,000 individuals. Sacramento River stocks had a heavier weighting in the central (89%) than in the northern (25%) fishery. Accuracy and precision increased for both fisheries as sample sizes increased and also were better for those estimates that were over 5%. Extrapolations from these estimates indicated that sample sizes of 2,320 and 2,869 would be required to fulfill coefficients of variation (SD/estimated contribution) of 20% with respective confidence intervals of 80 and 95% in stock groupings of the northern fishery. Similarly, sample sizes of 2,450 and 3,030 would be required in the central fishery. A concluding section noted that these investigations are part of an effort involving many agencies. The requirements for simulation preceding actual sampling of stock mixtures and for continued monitoring and development of baseline data sets were emphasized.

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96 pages

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  • Report No.: DOE/BP-23520-1
  • Grant Number: DE-A179-85BP23520
  • DOI: 10.2172/919534 | External Link
  • Office of Scientific & Technical Information Report Number: 919534
  • Archival Resource Key: ark:/67531/metadc901177

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Office of Scientific & Technical Information Technical Reports

Reports, articles and other documents harvested from the Office of Scientific and Technical Information.

Office of Scientific and Technical Information (OSTI) is the Department of Energy (DOE) office that collects, preserves, and disseminates DOE-sponsored research and development (R&D) results that are the outcomes of R&D projects or other funded activities at DOE labs and facilities nationwide and grantees at universities and other institutions.

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  • December 1, 1986

Added to The UNT Digital Library

  • Sept. 27, 2016, 1:39 a.m.

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Milner, George B. Genetic Stock Identification, Annual Report of Research 1986., report, December 1, 1986; United States. (digital.library.unt.edu/ark:/67531/metadc901177/: accessed October 21, 2017), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.