Isolation of Discrete Nanoparticle-DNA Conjugates for Plasmonic Applications

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Discrete DNA-gold nanoparticle conjugates with DNA lengths as short as 15 bases for both 5 nm and 20 nm gold particles have been purified by anion-exchange HPLC. Conjugates comprising short DNA (<40 bases) and large gold particles (>_ 20 nm) are difficult to purify by other means, and are potential substrates for plasmon coupling experiments. Conjugate purity is demonstrated by hybridizing complementary conjugates to form discrete structures, which are visualized by TEM.

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Alivisatos, Paul; Claridge, Shelley A.; Liang, Huiyang W.; Basu, Sourav Roger; Frechet, Jean M.J. & Alivisatos, A. Paul April 11, 2008.

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Discrete DNA-gold nanoparticle conjugates with DNA lengths as short as 15 bases for both 5 nm and 20 nm gold particles have been purified by anion-exchange HPLC. Conjugates comprising short DNA (<40 bases) and large gold particles (>_ 20 nm) are difficult to purify by other means, and are potential substrates for plasmon coupling experiments. Conjugate purity is demonstrated by hybridizing complementary conjugates to form discrete structures, which are visualized by TEM.

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11

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  • Journal Name: Nano Letters; Journal Volume: 8; Journal Issue: 4; Related Information: Journal Publication Date: April 2008

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  • Report No.: LBNL-194E
  • Grant Number: DE-AC02-05CH11231
  • Office of Scientific & Technical Information Report Number: 927872
  • Archival Resource Key: ark:/67531/metadc898507

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  • April 11, 2008

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  • Sept. 27, 2016, 1:39 a.m.

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  • Sept. 30, 2016, 6:37 p.m.

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Alivisatos, Paul; Claridge, Shelley A.; Liang, Huiyang W.; Basu, Sourav Roger; Frechet, Jean M.J. & Alivisatos, A. Paul. Isolation of Discrete Nanoparticle-DNA Conjugates for Plasmonic Applications, article, April 11, 2008; Berkeley, California. (digital.library.unt.edu/ark:/67531/metadc898507/: accessed September 22, 2017), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.