A cell nanoinjector based on carbon nanotubes

PDF Version Also Available for Download.

Description

Technologies for introducing molecules into living cells are vital for probing the physical properties and biochemical interactions that govern the cell's behavior. Here we report the development of a nanoscale cell injection system-termed the nanoinjector-that uses carbon nanotubes to deliver cargo into cells. A single multi-walled carbon nanotube attached to an atomic force microscope tip was functionalized with cargo via a disulfide-based linker. Penetration of cell membranes with this 'nanoneedle', followed by reductive cleavage of the disulfide bonds within the cell's interior, resulted in the release of cargo inside the cells. The capability of the nanoinjector was demonstrated by injection ... continued below

Creation Information

Chen, Xing; Kis, Andras; Zettl, Alex & Bertozzi, Carolyn R. January 30, 2007.

Context

This article is part of the collection entitled: Office of Scientific & Technical Information Technical Reports and was provided by UNT Libraries Government Documents Department to Digital Library, a digital repository hosted by the UNT Libraries. It has been viewed 16 times , with 4 in the last month . More information about this article can be viewed below.

Who

People and organizations associated with either the creation of this article or its content.

Sponsor

Publisher

Provided By

UNT Libraries Government Documents Department

Serving as both a federal and a state depository library, the UNT Libraries Government Documents Department maintains millions of items in a variety of formats. The department is a member of the FDLP Content Partnerships Program and an Affiliated Archive of the National Archives.

Contact Us

What

Descriptive information to help identify this article. Follow the links below to find similar items on the Digital Library.

Description

Technologies for introducing molecules into living cells are vital for probing the physical properties and biochemical interactions that govern the cell's behavior. Here we report the development of a nanoscale cell injection system-termed the nanoinjector-that uses carbon nanotubes to deliver cargo into cells. A single multi-walled carbon nanotube attached to an atomic force microscope tip was functionalized with cargo via a disulfide-based linker. Penetration of cell membranes with this 'nanoneedle', followed by reductive cleavage of the disulfide bonds within the cell's interior, resulted in the release of cargo inside the cells. The capability of the nanoinjector was demonstrated by injection of protein-coated quantum dots into live human cells. Single-particle tracking was employed to characterize the diffusion dynamics of injected quantum dots in the cytosol. This new technique causes no discernible membrane or cell damage, and can deliver a discrete number of molecules to the cell's interior without the requirement of a carrier solvent.

Source

  • Journal Name: Proceedings of the National Academy ofSciences; Journal Volume: 104; Journal Issue: 20; Related Information: Journal Publication Date: 05/15/2007

Language

Item Type

Identifier

Unique identifying numbers for this article in the Digital Library or other systems.

  • Report No.: LBNL--62619
  • Grant Number: DE-AC02-05CH11231
  • Office of Scientific & Technical Information Report Number: 929020
  • Archival Resource Key: ark:/67531/metadc898410

Collections

This article is part of the following collection of related materials.

Office of Scientific & Technical Information Technical Reports

Reports, articles and other documents harvested from the Office of Scientific and Technical Information.

Office of Scientific and Technical Information (OSTI) is the Department of Energy (DOE) office that collects, preserves, and disseminates DOE-sponsored research and development (R&D) results that are the outcomes of R&D projects or other funded activities at DOE labs and facilities nationwide and grantees at universities and other institutions.

What responsibilities do I have when using this article?

When

Dates and time periods associated with this article.

Creation Date

  • January 30, 2007

Added to The UNT Digital Library

  • Sept. 27, 2016, 1:39 a.m.

Description Last Updated

  • Oct. 31, 2016, 3:49 p.m.

Usage Statistics

When was this article last used?

Yesterday: 1
Past 30 days: 4
Total Uses: 16

Interact With This Article

Here are some suggestions for what to do next.

Start Reading

PDF Version Also Available for Download.

Citations, Rights, Re-Use

Chen, Xing; Kis, Andras; Zettl, Alex & Bertozzi, Carolyn R. A cell nanoinjector based on carbon nanotubes, article, January 30, 2007; Berkeley, California. (digital.library.unt.edu/ark:/67531/metadc898410/: accessed January 21, 2018), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.