Bacillus atrophaeus Outer Spore Coat Assembly and Ultrastructure

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Our previous atomic force microscopy (AFM) studies successfully visualized native Bacillus atrophaeus spore coat ultrastructure and surface morphology. We have shown that the outer spore coat surface is formed by a crystalline array of {approx}11 nm thick rodlets, having a periodicity of {approx}8 nm. We present here further AFM ultrastructural investigations of air-dried and fully hydrated spore surface architecture. In the rodlet layer, planar and point defects, as well as domain boundaries, similar to those described for inorganic and macromolecular crystals, were identified. For several Bacillus species, rodlet structure assembly and architectural variation appear to be a consequence of species-specific ... continued below

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PDF-file: 36 pages; size: 3.7 Mbytes

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Plomp, M; Leighton, T J; Wheeler, K E; Pitesky, M E & Malkin, A J November 21, 2005.

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Our previous atomic force microscopy (AFM) studies successfully visualized native Bacillus atrophaeus spore coat ultrastructure and surface morphology. We have shown that the outer spore coat surface is formed by a crystalline array of {approx}11 nm thick rodlets, having a periodicity of {approx}8 nm. We present here further AFM ultrastructural investigations of air-dried and fully hydrated spore surface architecture. In the rodlet layer, planar and point defects, as well as domain boundaries, similar to those described for inorganic and macromolecular crystals, were identified. For several Bacillus species, rodlet structure assembly and architectural variation appear to be a consequence of species-specific nucleation and crystallization mechanisms that regulate the formation of the outer spore coat. We propose a unifying mechanism for nucleation and self-assembly of this crystalline layer on the outer spore coat surface.

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PDF-file: 36 pages; size: 3.7 Mbytes

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  • Journal Name: Langmuir, vol. 21, no. 23, October 5, 2005, pp. 10710-10716; Journal Volume: 21; Journal Issue: 23

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  • Report No.: UCRL-JRNL-217296
  • Grant Number: W-7405-ENG-48
  • Office of Scientific & Technical Information Report Number: 936440
  • Archival Resource Key: ark:/67531/metadc898200

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Office of Scientific & Technical Information Technical Reports

Reports, articles and other documents harvested from the Office of Scientific and Technical Information.

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  • November 21, 2005

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  • Sept. 27, 2016, 1:39 a.m.

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  • Dec. 2, 2016, 8:30 p.m.

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Plomp, M; Leighton, T J; Wheeler, K E; Pitesky, M E & Malkin, A J. Bacillus atrophaeus Outer Spore Coat Assembly and Ultrastructure, article, November 21, 2005; Livermore, California. (digital.library.unt.edu/ark:/67531/metadc898200/: accessed December 14, 2017), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.