Gel Electrophoresis of Gold-DNA Nano-Conjugates

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Single stranded DNA of different lengths and different amounts was attached to colloidal phosphine stabilized Au nanoparticles. The resulting conjugates were investigated in detail by a gel electrophoresis study based on 1200 gels. We demonstrate how these experiments help to understand the binding of DNA to Au particles. In particular we compare specific attachment of DNA via gold-thiol bonds with nonspecific adsorption of DNA. The maximum number of DNA molecules that can be bound per particle was determined. We also compare several methods to used gel electrophoresis for investigating the effective diameter of DNA-Au conjugates, such as using a calibration ... continued below

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Pellegrino, T.; Sperling, R.A.; Alivisatos, A.P. & Parak, W.J. January 10, 2006.

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Single stranded DNA of different lengths and different amounts was attached to colloidal phosphine stabilized Au nanoparticles. The resulting conjugates were investigated in detail by a gel electrophoresis study based on 1200 gels. We demonstrate how these experiments help to understand the binding of DNA to Au particles. In particular we compare specific attachment of DNA via gold-thiol bonds with nonspecific adsorption of DNA. The maximum number of DNA molecules that can be bound per particle was determined. We also compare several methods to used gel electrophoresis for investigating the effective diameter of DNA-Au conjugates, such as using a calibration curve of particles with known diameters and Ferguson plots.

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  • Journal Name: Journal of Biomedicine and Biotechnology; Journal Volume: 2007; Journal Issue: 10; Related Information: Journal Publication Date: 2007

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  • Report No.: LBNL--61541
  • Grant Number: DE-AC02-05CH11231
  • Office of Scientific & Technical Information Report Number: 929759
  • Archival Resource Key: ark:/67531/metadc897187

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Office of Scientific & Technical Information Technical Reports

Reports, articles and other documents harvested from the Office of Scientific and Technical Information.

Office of Scientific and Technical Information (OSTI) is the Department of Energy (DOE) office that collects, preserves, and disseminates DOE-sponsored research and development (R&D) results that are the outcomes of R&D projects or other funded activities at DOE labs and facilities nationwide and grantees at universities and other institutions.

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  • January 10, 2006

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  • Sept. 27, 2016, 1:39 a.m.

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Pellegrino, T.; Sperling, R.A.; Alivisatos, A.P. & Parak, W.J. Gel Electrophoresis of Gold-DNA Nano-Conjugates, article, January 10, 2006; United States. (digital.library.unt.edu/ark:/67531/metadc897187/: accessed October 21, 2017), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.