Response of Desulfovibrio vulgaris to Alkaline Stress Page: 2 of 36
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The response of exponentially growing Desulfovibrio vulgaris Hildenborough (DvH) to pH 10
stress was studied using oligonucleotide microarrays and a study set of mutants deleted in genes
suggested by microarray data to be involved in alkaline stress response. These data showed that
the response of D. vulgaris to increased pH is generally similar to E. coi, but apparently
controlled by unique regulatory circuits since the alternative sigma factors (sigma S and E)
contributing to this stress response in E. coi appear to be absent in D. vulgaris. Genes
previously reported to be up-regulated in E. coi were up-regulated in D. vulgaris, including
three ATPase genes and a tryptophan synthase gene. Transcription of chaperone and protease
genes (ATP-dependent Clp and La proteases, and DnaK) were also elevated in D. vulgaris. As
in E. co/i, genes involved in flagella synthesis were down-regulated. The transcriptional data
also identified regulators, distinct from sigma S and E, likely part of a DvH-specific stress
response system. Characterization of a study set of mutants deleted in genes implicated in
alkaline stress response confirmed a protective involvement of sodium/proton antiporter NhaC-2,
tryptophanase A, and two putative regulators/hi stidine kinases DVU0331 and DVU2580.
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Stolyar, S.; He, Q.; He, Z.; Yang, Z.; Borglin, S.E.; Joyner, D. et al. Response of Desulfovibrio vulgaris to Alkaline Stress, article, November 30, 2007; United States. (digital.library.unt.edu/ark:/67531/metadc896301/m1/2/: accessed January 20, 2019), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.