Amphotericin B induced interdigitation of apolipoprotein stabilized nanodisk bilayers

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Amphotericin B nanodisks (AMB-ND) are ternary complexes of AMB, phospholipid (PL) and apolipoprotein organized as discrete nanometer scale disk-shaped bilayers. In gel filtration chromatography experiments, empty ND lacking AMB elute as a single population of particles with a molecular weight in the range of 200 kDa. AMB-ND formulated at a 4:1 PL:AMB weight ratio, separated into two peaks. Peak 1 eluted at the position of control ND lacking AMB while the second peak, containing all of the AMB present in the original sample, eluted in the void volume. When ND prepared with increased AMB (1:1 phospholipid:AMB molar ratio) were subjected ... continued below

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12 p. (0.2 MB)

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Nguyen, T; Weers, P M; Sulchek, T; Hoeprich, P D & Ryan, R O December 7, 2006.

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Amphotericin B nanodisks (AMB-ND) are ternary complexes of AMB, phospholipid (PL) and apolipoprotein organized as discrete nanometer scale disk-shaped bilayers. In gel filtration chromatography experiments, empty ND lacking AMB elute as a single population of particles with a molecular weight in the range of 200 kDa. AMB-ND formulated at a 4:1 PL:AMB weight ratio, separated into two peaks. Peak 1 eluted at the position of control ND lacking AMB while the second peak, containing all of the AMB present in the original sample, eluted in the void volume. When ND prepared with increased AMB (1:1 phospholipid:AMB molar ratio) were subjected to gel filtration chromatography, an increased proportion of phospholipid and apolipoprotein were recovered in the void volume with the AMB. Prior to gel filtration the AMB-ND sample could be passed through a 0.22 {micro}m filter without loss of AMB while the voided material was lost. Native gel electrophoresis studies corroborated the gel permeation chromatography data. Far UV circular dichroism analyses revealed that apoA-I associated with AMB-ND denatures at a lower guanidine HCl concentration than apoA-I associated with ND lacking AMB. Atomic force microscopy revealed that AMB induces compression of the ND bilayer thickness consistent with bilayer interdigitation, a phenomenon that is likely related to the ability of AMB to induce pore formation in susceptible membranes.

Physical Description

12 p. (0.2 MB)

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PDF-file: 12 pages; size: 0.2 Mbytes

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  • Journal Name: Biochimica et Biophysica Acta, vol. 1778, N/A, October 16, 2007, pp. 303-312; Journal Volume: 1778

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  • Report No.: UCRL-JRNL-226688
  • Grant Number: W-7405-ENG-48
  • Office of Scientific & Technical Information Report Number: 936959
  • Archival Resource Key: ark:/67531/metadc894885

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Office of Scientific & Technical Information Technical Reports

Reports, articles and other documents harvested from the Office of Scientific and Technical Information.

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  • December 7, 2006

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  • Sept. 27, 2016, 1:39 a.m.

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  • April 13, 2017, 3:58 p.m.

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Nguyen, T; Weers, P M; Sulchek, T; Hoeprich, P D & Ryan, R O. Amphotericin B induced interdigitation of apolipoprotein stabilized nanodisk bilayers, article, December 7, 2006; Livermore, California. (digital.library.unt.edu/ark:/67531/metadc894885/: accessed November 23, 2017), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.