Performance of Molecular Inversion Probes (MIP) in Allele CopyNumber Determination

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Description

We have developed a new protocol for using MolecularInversion Probes (MIP) to accurately and specifically measure allele copynumber (ACN). The new protocol provides for significant improvementsincluding the reduction of input DNA (from 2?g) by more than 25 fold (to75ng total genomic DNA), higher overall precision resulting in one orderof magnitude lower false positive rate, and greater dynamic range withaccurate absolute copy number up to 60 copies.

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Wang, Yuker; Moorhead, Martin; Karlin-Neumann, George; Wang,Nicolas J.; Ireland, James; Lin, Steven et al. May 14, 2007.

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Description

We have developed a new protocol for using MolecularInversion Probes (MIP) to accurately and specifically measure allele copynumber (ACN). The new protocol provides for significant improvementsincluding the reduction of input DNA (from 2?g) by more than 25 fold (to75ng total genomic DNA), higher overall precision resulting in one orderof magnitude lower false positive rate, and greater dynamic range withaccurate absolute copy number up to 60 copies.

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  • Journal Name: Genome Biology; Journal Volume: 8; Journal Issue: 11; Related Information: Journal Publication Date: 11/20/2007

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  • Report No.: LBNL--62691
  • Grant Number: DE-AC02-05CH11231
  • DOI: 10.1186/gb-2007-8-11-r246 | External Link
  • Office of Scientific & Technical Information Report Number: 932480
  • Archival Resource Key: ark:/67531/metadc894308

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Office of Scientific & Technical Information Technical Reports

Reports, articles and other documents harvested from the Office of Scientific and Technical Information.

Office of Scientific and Technical Information (OSTI) is the Department of Energy (DOE) office that collects, preserves, and disseminates DOE-sponsored research and development (R&D) results that are the outcomes of R&D projects or other funded activities at DOE labs and facilities nationwide and grantees at universities and other institutions.

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  • May 14, 2007

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  • Sept. 27, 2016, 1:39 a.m.

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Wang, Yuker; Moorhead, Martin; Karlin-Neumann, George; Wang,Nicolas J.; Ireland, James; Lin, Steven et al. Performance of Molecular Inversion Probes (MIP) in Allele CopyNumber Determination, article, May 14, 2007; United States. (digital.library.unt.edu/ark:/67531/metadc894308/: accessed December 13, 2017), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.