Genomic analysis of the symbiotic marine crenarchaeon, Cenarchaeumsymbiosum

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Crenarchaea are ubiquitous and abundant microbial constituents of soils, sediments, lakes and ocean waters, yet relatively little is known about their fundamental evolutionary, ecological, and physiological properties. To better describe the ubiquitous nonthermophilic Crenarchaea, we analyzed the genome sequence of one representative, the uncultivated sponge symbiont, Cenarchaeum symbiosum. C. symbiosum genotypes coinhabiting the same host partitioned into two dominant populations, corresponding to previously described a- and b-type ribosomal RNA variants. Although synthetic, overlapping a- and b-type ribotypes harbored significant genetic variability. A single tiling path comprising the dominant a-type genotype was assembled, and used to explore the biological properties of ... continued below

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Hallam, Steven J.; Konstantinidis, Konstantinos T.; Brochier,Celine; Putnam, Nik; Schleper, Christa; Watanabe, Yoh-ichi et al. June 24, 2006.

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Crenarchaea are ubiquitous and abundant microbial constituents of soils, sediments, lakes and ocean waters, yet relatively little is known about their fundamental evolutionary, ecological, and physiological properties. To better describe the ubiquitous nonthermophilic Crenarchaea, we analyzed the genome sequence of one representative, the uncultivated sponge symbiont, Cenarchaeum symbiosum. C. symbiosum genotypes coinhabiting the same host partitioned into two dominant populations, corresponding to previously described a- and b-type ribosomal RNA variants. Although synthetic, overlapping a- and b-type ribotypes harbored significant genetic variability. A single tiling path comprising the dominant a-type genotype was assembled, and used to explore the biological properties of C. symbiosum and its planktonic relatives. Out of a total of 2,066 predicted open reading frames, 36% were more highly conserved with other Archaea. The remainder partitioned between bacteria (18%), eukaryotes (1.5%) and viruses (0.1%). A total of 525 open reading frames were more highly conserved with sequences derived from marine environmental genomic surveys, most probably representing orthologous genes found in free-living planktonic Crenarchaea. The remaining genes partitioned between functional RNAs (2.4%), and hypotheticals (42%) with limited homology to known functional genes. The latter category likely contains genes specifically involved in mediated archaeal-sponge symbiosis. Phylogenetic analyses placed C. symbiosum as a basal crenarchaeon, sharing specific genomic features in common with either Crenarchaea, Euryarchaea, or both. The genome sequence of C. symbiosum reflect a unique and unusual evolutionary, physiological, and ecological history, one remarkably distinct from that of any other previously known microbial lineage.

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  • Journal Name: Proceedings of the National Academy ofSciences; Journal Volume: 103; Journal Issue: 48; Related Information: Journal Publication Date: November 28,2006

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  • Report No.: LBNL--62943
  • Grant Number: DE-AC02-05CH11231
  • Office of Scientific & Technical Information Report Number: 927384
  • Archival Resource Key: ark:/67531/metadc893497

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  • June 24, 2006

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  • Sept. 27, 2016, 1:39 a.m.

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  • Sept. 29, 2016, 2:47 p.m.

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Hallam, Steven J.; Konstantinidis, Konstantinos T.; Brochier,Celine; Putnam, Nik; Schleper, Christa; Watanabe, Yoh-ichi et al. Genomic analysis of the symbiotic marine crenarchaeon, Cenarchaeumsymbiosum, article, June 24, 2006; Berkeley, California. (digital.library.unt.edu/ark:/67531/metadc893497/: accessed August 23, 2017), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.