THE INHIBITION OF DEOXYRIBONUCLEASS I BY HYDEOXY-PIPHENYLS

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DNA extracted with certain commercial brands of phenol is resistant to hydrolysis by the endonuclease DNAase I, while DNA extracted with other brands, or prepared by sodium chloride extraction, is susceptible to hydrolysis. The agent responsible for inhibition has been shown to be an oxidation product produced in some phenols. The inhibitor has been separated from other impurities in phenol by paper chromatography, and, by means of infrared and ultraviolet spectroscopy, it has been identified as o-hydroxybiphenyl. The kinetics of inhibition have been studied, and it was found that inhibition arises from direct action on the DNA rather than on ... continued below

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57 p.

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Gottesfeld, J.M.; Adams, N.H.; El-Badry, A.M.; Moses, V. & Calvin,M. September 1, 1970.

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Description

DNA extracted with certain commercial brands of phenol is resistant to hydrolysis by the endonuclease DNAase I, while DNA extracted with other brands, or prepared by sodium chloride extraction, is susceptible to hydrolysis. The agent responsible for inhibition has been shown to be an oxidation product produced in some phenols. The inhibitor has been separated from other impurities in phenol by paper chromatography, and, by means of infrared and ultraviolet spectroscopy, it has been identified as o-hydroxybiphenyl. The kinetics of inhibition have been studied, and it was found that inhibition arises from direct action on the DNA rather than on the enzyme. Several hydroxybiphenyls and related compounds have been tested for inhibition, and a theory of molecular structure versus inhibitory effectiveness is suggested from this data. From studies on the chemical reversal of inhibition, as well as from ultraviolet spectral studies (in both absorption and circular dichroism), it appears that the mode of action of the inhibitors is hydrogen bonding to, and intercalation between, the basis of the nucleic acid.

Physical Description

57 p.

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  • Report No.: UCRL--20108
  • Grant Number: DE-AC02-05CH11231
  • DOI: 10.2172/928496 | External Link
  • Office of Scientific & Technical Information Report Number: 928496
  • Archival Resource Key: ark:/67531/metadc893351

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Office of Scientific & Technical Information Technical Reports

Reports, articles and other documents harvested from the Office of Scientific and Technical Information.

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Creation Date

  • September 1, 1970

Added to The UNT Digital Library

  • Sept. 27, 2016, 1:39 a.m.

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  • Nov. 3, 2016, 7:57 p.m.

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Gottesfeld, J.M.; Adams, N.H.; El-Badry, A.M.; Moses, V. & Calvin,M. THE INHIBITION OF DEOXYRIBONUCLEASS I BY HYDEOXY-PIPHENYLS, report, September 1, 1970; Berkeley, California. (digital.library.unt.edu/ark:/67531/metadc893351/: accessed November 23, 2017), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.