Application of NMR Methods to Identify Detection Reagents for Use in the Development of Robust Nanosensors

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Nuclear Magnetic Resonance (NMR) spectroscopy is a powerful technique for studying bi-molecular interactions at the atomic scale. Our NMR lab is involved in the identification of small molecules, or ligands that bind to target protein receptors, such as tetanus (TeNT) and botulinum (BoNT) neurotoxins, anthrax proteins and HLA-DR10 receptors on non-Hodgkin's lymphoma cancer cells. Once low affinity binders are identified, they can be linked together to produce multidentate synthetic high affinity ligands (SHALs) that have very high specificity for their target protein receptors. An important nanotechnology application for SHALs is their use in the development of robust chemical sensors or … continued below

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Cosman, M; Krishnan, V V & Balhorn, R April 29, 2004.

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Nuclear Magnetic Resonance (NMR) spectroscopy is a powerful technique for studying bi-molecular interactions at the atomic scale. Our NMR lab is involved in the identification of small molecules, or ligands that bind to target protein receptors, such as tetanus (TeNT) and botulinum (BoNT) neurotoxins, anthrax proteins and HLA-DR10 receptors on non-Hodgkin's lymphoma cancer cells. Once low affinity binders are identified, they can be linked together to produce multidentate synthetic high affinity ligands (SHALs) that have very high specificity for their target protein receptors. An important nanotechnology application for SHALs is their use in the development of robust chemical sensors or biochips for the detection of pathogen proteins in environmental samples or body fluids. Here, we describe a recently developed NMR competition assay based on transferred nuclear Overhauser effect spectroscopy (trNOESY) that enables the identification of sets of ligands that bind to the same site, or a different site, on the surface of TeNT fragment C (TetC) than a known ''marker'' ligand, doxorubicin. Using this assay, we can identify the optimal pairs of ligands to be linked together for creating detection reagents, as well as estimate the relative binding constants for ligands competing for the same site.

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PDF-file: 42 pages; size: 1.8 Mbytes

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  • Report No.: UCRL-BOOK-203945
  • Grant Number: W-7405-ENG-48
  • Office of Scientific & Technical Information Report Number: 883828
  • Archival Resource Key: ark:/67531/metadc892750

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  • April 29, 2004

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  • Sept. 23, 2016, 2:42 p.m.

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  • Dec. 2, 2016, 8:14 p.m.

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Cosman, M; Krishnan, V V & Balhorn, R. Application of NMR Methods to Identify Detection Reagents for Use in the Development of Robust Nanosensors, book, April 29, 2004; Livermore, California. (https://digital.library.unt.edu/ark:/67531/metadc892750/: accessed October 12, 2026), University of North Texas Libraries, UNT Digital Library, https://digital.library.unt.edu; crediting UNT Libraries Government Documents Department.

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