Physiomics Array: A Platform for Genome Research and Cultivation of Difficult-to-Cultivate Microorganisms Final Technical Report

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A scalable array technology for parametric control of high-throughput cell cultivations is demonstrated. The technology makes use of commercial printed circuit board (PCB) technology, integrated circuit sensors, and an electrochemical gas generation system. We present results for an array of eight 250 μl microbioreactors. Each bioreactor contains an independently addressable suite that provides closed-loop temperature control, generates feed gas electrochemically, and continuously monitors optical density. The PCB technology allows for the assembly of additional off-the-shelf components into the microbioreactor array; we demonstrate the use of a commercial ISFET chip to continuously monitor culture pH. The electrochemical dosing system provides a ... continued below

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Keasling, Jay D. July 10, 2006.

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A scalable array technology for parametric control of high-throughput cell cultivations is demonstrated. The technology makes use of commercial printed circuit board (PCB) technology, integrated circuit sensors, and an electrochemical gas generation system. We present results for an array of eight 250 μl microbioreactors. Each bioreactor contains an independently addressable suite that provides closed-loop temperature control, generates feed gas electrochemically, and continuously monitors optical density. The PCB technology allows for the assembly of additional off-the-shelf components into the microbioreactor array; we demonstrate the use of a commercial ISFET chip to continuously monitor culture pH. The electrochemical dosing system provides a powerful paradigm for reproducible gas delivery to high-density arrays of microreactors. We have scaled the technology to a standard 96-well format and have constructed a system that could be easily assembled.

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  • Report No.: DOE/ER/63144-1
  • Grant Number: FG02-01ER63144
  • DOI: 10.2172/886023 | External Link
  • Office of Scientific & Technical Information Report Number: 886023
  • Archival Resource Key: ark:/67531/metadc891975

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Office of Scientific & Technical Information Technical Reports

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  • July 10, 2006

Added to The UNT Digital Library

  • Sept. 23, 2016, 2:42 p.m.

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  • Nov. 30, 2016, 4:46 p.m.

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Keasling, Jay D. Physiomics Array: A Platform for Genome Research and Cultivation of Difficult-to-Cultivate Microorganisms Final Technical Report, report, July 10, 2006; United States. (digital.library.unt.edu/ark:/67531/metadc891975/: accessed October 19, 2017), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.