Droplet-Based Segregation and Extraction of Concentrated Samples

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Microfluidic analysis often requires sample concentration and separation techniques to isolate and detect analytes of interest. Complex or scarce samples may also require an orthogonal separation and detection method or off-chip analysis to confirm results. To perform these additional steps, the concentrated sample plug must be extracted from the primary microfluidic channel with minimal sample loss and dilution. We investigated two extraction techniques; injection of immiscible fluid droplets into the sample stream (''capping'''') and injection of the sample into an immiscible fluid stream (''extraction''). From our results we conclude that capping is the more effective partitioning technique. Furthermore, this functionality ... continued below

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6 p. (0.5 MB)

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Buie, C R; Buckley, P; Hamilton, J; Ness, K D & Rose, K A February 23, 2007.

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Microfluidic analysis often requires sample concentration and separation techniques to isolate and detect analytes of interest. Complex or scarce samples may also require an orthogonal separation and detection method or off-chip analysis to confirm results. To perform these additional steps, the concentrated sample plug must be extracted from the primary microfluidic channel with minimal sample loss and dilution. We investigated two extraction techniques; injection of immiscible fluid droplets into the sample stream (''capping'''') and injection of the sample into an immiscible fluid stream (''extraction''). From our results we conclude that capping is the more effective partitioning technique. Furthermore, this functionality enables additional off-chip post-processing procedures such as DNA/RNA microarray analysis, realtime polymerase chain reaction (RT-PCR), and culture growth to validate chip performance.

Physical Description

6 p. (0.5 MB)

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PDF-file: 6 pages; size: 0.5 Mbytes

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  • Presented at: NSTI Nanotech 2007, Santa Clara, CA, United States, May 20 - May 24, 2007

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  • Report No.: UCRL-PROC-228474
  • Grant Number: W-7405-ENG-48
  • Office of Scientific & Technical Information Report Number: 909630
  • Archival Resource Key: ark:/67531/metadc891454

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Office of Scientific & Technical Information Technical Reports

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  • February 23, 2007

Added to The UNT Digital Library

  • Sept. 22, 2016, 2:13 a.m.

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  • April 17, 2017, 12:56 p.m.

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Buie, C R; Buckley, P; Hamilton, J; Ness, K D & Rose, K A. Droplet-Based Segregation and Extraction of Concentrated Samples, article, February 23, 2007; Livermore, California. (digital.library.unt.edu/ark:/67531/metadc891454/: accessed July 17, 2018), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.