Chemical and biological differentiation of three human breast cancer cell types using time-of-flight secondary ion mass spectrometry (TOF-SIMS)

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We use Time-of-Flight Secondary Ion Mass Spectrometry (TOF-SIMS) to image and classify individual cells based on their characteristic mass spectra. Using statistical data reduction on the large data sets generated during TOF-SIMS analysis, similar biological materials can be differentiated based on a combination of small changes in protein expression, metabolic activity and cell structure. We apply this powerful technique to image and differentiate three carcinoma-derived human breast cancer cell lines (MCF-7, T47D and MDA-MB-231). In homogenized cells, we show the ability to differentiate the cell types as well as cellular compartments (cytosol, nuclear and membrane). These studies illustrate the capacity ... continued below

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Kulp, K S; Berman, E F; Knize, M G; Shattuck, D L; Nelson, E J; Wu, L et al. January 9, 2006.

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We use Time-of-Flight Secondary Ion Mass Spectrometry (TOF-SIMS) to image and classify individual cells based on their characteristic mass spectra. Using statistical data reduction on the large data sets generated during TOF-SIMS analysis, similar biological materials can be differentiated based on a combination of small changes in protein expression, metabolic activity and cell structure. We apply this powerful technique to image and differentiate three carcinoma-derived human breast cancer cell lines (MCF-7, T47D and MDA-MB-231). In homogenized cells, we show the ability to differentiate the cell types as well as cellular compartments (cytosol, nuclear and membrane). These studies illustrate the capacity of TOF-SIMS to characterize individual cells by chemical composition, which could ultimately be applied to detect and identify single aberrant cells within a normal cell population. Ultimately, we anticipate characterizing rare chemical changes that may provide clues to single cell progression within carcinogenic and metastatic pathways.

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PDF-file: 29 pages; size: 0.3 Mbytes

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  • Journal Name: Analytical Chemistry, vol. 78, no. 11, June 1, 2006, pp. 3651-3658

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  • Report No.: UCRL-JRNL-218036
  • Grant Number: W-7405-ENG-48
  • Office of Scientific & Technical Information Report Number: 898469
  • Archival Resource Key: ark:/67531/metadc890505

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  • January 9, 2006

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  • Sept. 22, 2016, 2:13 a.m.

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  • Nov. 22, 2016, 10:21 p.m.

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Kulp, K S; Berman, E F; Knize, M G; Shattuck, D L; Nelson, E J; Wu, L et al. Chemical and biological differentiation of three human breast cancer cell types using time-of-flight secondary ion mass spectrometry (TOF-SIMS), article, January 9, 2006; Livermore, California. (digital.library.unt.edu/ark:/67531/metadc890505/: accessed July 21, 2018), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.