Functional Stability Of A Mixed Microbial Consortia Producing PHA From Waste Carbon Sources

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Polyhydroxyalkanoates (PHAs), naturally-occurring biological polyesters that are microbially synthesized from a myriad of carbon sources, can be utilized as biodegradable substitutes for petroleum-derived thermoplastics. However, current PHA commercialization schemes are limited by high feedstock costs, the requirement for aseptic reactors, and high separation and purification costs. Bacteria indigenous to municipal waste streams can accumulate large quantities of PHA under environmentally controlled conditions; hence, a potentially more environmentally-effective method of production would utilize these consortia to produce PHAs from inexpensive waste carbon sources. In this study, PHA production was accomplished in sequencing batch bioreactors utilizing mixed microbial consortia from municipal activated ... continued below

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Thompson, David N.; Coats, Erik R.; Smith, William A.; Loge, Frank J. & Wolcott, Michael P. April 1, 2006.

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Polyhydroxyalkanoates (PHAs), naturally-occurring biological polyesters that are microbially synthesized from a myriad of carbon sources, can be utilized as biodegradable substitutes for petroleum-derived thermoplastics. However, current PHA commercialization schemes are limited by high feedstock costs, the requirement for aseptic reactors, and high separation and purification costs. Bacteria indigenous to municipal waste streams can accumulate large quantities of PHA under environmentally controlled conditions; hence, a potentially more environmentally-effective method of production would utilize these consortia to produce PHAs from inexpensive waste carbon sources. In this study, PHA production was accomplished in sequencing batch bioreactors utilizing mixed microbial consortia from municipal activated sludge as inoculum, in cultures grown on real wastewaters. PHA production averaged 85%, 53%, and 10% of the cell dry weight from methanol-enriched pulp-and-paper mill foul condensate, fermented municipal primary solids, and biodiesel wastewater, respectively. The PHA-producing microbial consortia were examined to explore the microbial community changes that occurred during reactor operations, employing denaturing gradient gel electrophoresis (DGGE) of 16S-rDNA from PCR-amplified DNA extracts. Distinctly different communities were observed both between and within wastewaters following enrichment. More importantly, stable functions were maintained despite the differing and contrasting microbial populations.

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  • 28th Symposium on Biotechnology for Fuels and Chemicals,Nashville, TN,04/30/2006,05/03/2006

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  • Report No.: INL/CON-05-01051
  • Grant Number: DE-AC07-99ID-13727
  • Office of Scientific & Technical Information Report Number: 911930
  • Archival Resource Key: ark:/67531/metadc888799

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  • April 1, 2006

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  • Sept. 22, 2016, 2:13 a.m.

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  • Nov. 7, 2016, 4:13 p.m.

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Thompson, David N.; Coats, Erik R.; Smith, William A.; Loge, Frank J. & Wolcott, Michael P. Functional Stability Of A Mixed Microbial Consortia Producing PHA From Waste Carbon Sources, article, April 1, 2006; [Idaho Falls, Idaho]. (digital.library.unt.edu/ark:/67531/metadc888799/: accessed October 18, 2018), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.