Short Peptides Enhance Single Cell Adhesion and Viability onMicroarrays

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Single cell patterning holds important implications forbiology, biochemistry, biotechnology, medicine, and bioinformatics. Thechallenge for single cell patterning is to produce small islands hostingonly single cells and retaining their viability for a prolonged period oftime. This study demonstrated a surface engineering approach that uses acovalently bound short peptide as a mediator to pattern cells withimproved single cell adhesion and prolonged cellular viabilityon goldpatterned SiO2 substrates. The underlying hypothesis is that celladhesion is regulated bythe type, availability, and stability ofeffective cell adhesion peptides, and thus covalently bound shortpeptides would promote cell spreading and, thus, single cell adhesion andviability. The effectiveness of this ... continued below

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Veiseh, Mandana; Veiseh, Omid; Martin, Michael C.; Asphahani,Fareid & Zhang, Miqin January 19, 2007.

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Single cell patterning holds important implications forbiology, biochemistry, biotechnology, medicine, and bioinformatics. Thechallenge for single cell patterning is to produce small islands hostingonly single cells and retaining their viability for a prolonged period oftime. This study demonstrated a surface engineering approach that uses acovalently bound short peptide as a mediator to pattern cells withimproved single cell adhesion and prolonged cellular viabilityon goldpatterned SiO2 substrates. The underlying hypothesis is that celladhesion is regulated bythe type, availability, and stability ofeffective cell adhesion peptides, and thus covalently bound shortpeptides would promote cell spreading and, thus, single cell adhesion andviability. The effectiveness of this approach and the underlyingmechanism for the increased probability of single cell adhesion andprolonged cell viability by short peptides were studied by comparingcellular behavior of human umbilical cord vein endothelial cells on threemodelsurfaces whose gold electrodes were immobilized with fibronectin,physically adsorbed Arg-Glu-Asp-Val-Tyr, and covalently boundLys-Arg-Glu-Asp-Val-Tyr, respectively. The surface chemistry and bindingproperties were characterized by reflectance Fourier transform infraredspectroscopy. Both short peptides were superior to fibronectin inproducing adhesion of only single cells, whereas the covalently boundpeptide also reduced apoptosis and necrosisof adhered cells. Controllingcell spreading by peptide binding domains to regulate apoptosis andviability represents a fundamental mechanism in cell-materialsinteraction and provides an effective strategy in engineering arrays ofsingle cells.

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  • Journal Name: Langmuir; Journal Volume: 23; Journal Issue: 8; Related Information: Journal Publication Date: April 10,2007

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  • Report No.: LBNL--62720
  • Grant Number: DE-AC02-05CH11231
  • Grant Number: NSF:NSF-EEC 9529161
  • Grant Number: NIHR01GM075095
  • DOI: 10.1021/la062849k | External Link
  • Office of Scientific & Technical Information Report Number: 918638
  • Archival Resource Key: ark:/67531/metadc887991

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  • January 19, 2007

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  • Sept. 22, 2016, 2:13 a.m.

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  • Sept. 29, 2016, 7:01 p.m.

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Veiseh, Mandana; Veiseh, Omid; Martin, Michael C.; Asphahani,Fareid & Zhang, Miqin. Short Peptides Enhance Single Cell Adhesion and Viability onMicroarrays, article, January 19, 2007; Berkeley, California. (digital.library.unt.edu/ark:/67531/metadc887991/: accessed August 18, 2017), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.