HYDROCARBON-DEGRADING BACTERIA AND SURFACTANT ACTIVITY

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Fate of benzene ethylbenzene toluene xylenes (BTEX) compounds through biodegradation was investigated using two different bacteria, Ralstonia picketti (BP-20) and Alcaligenes piechaudii (CZOR L-1B). These bacteria were isolated from extremely polluted petroleum hydrocarbon contaminated soils. PCR and Fatty Acid Methyl Ester (FAME) were used to identify the isolates. Biodegradation was measured using each organism individually and in combination. Both bacteria were shown to degrade each of the BTEX compounds. Alcaligenes piechaudii biodegraded BTEXs more efficiently while mixed with BP-20 and individually. Biosurfactant production was observed by culture techniques. In addition 3-hydroxy fatty acids, important in biosurfactant production, was observed by ... continued below

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Brigmon, R; Topher Berry, T; Grazyna A. Plaza, G & jacek Wypych, j August 15, 2006.

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Fate of benzene ethylbenzene toluene xylenes (BTEX) compounds through biodegradation was investigated using two different bacteria, Ralstonia picketti (BP-20) and Alcaligenes piechaudii (CZOR L-1B). These bacteria were isolated from extremely polluted petroleum hydrocarbon contaminated soils. PCR and Fatty Acid Methyl Ester (FAME) were used to identify the isolates. Biodegradation was measured using each organism individually and in combination. Both bacteria were shown to degrade each of the BTEX compounds. Alcaligenes piechaudii biodegraded BTEXs more efficiently while mixed with BP-20 and individually. Biosurfactant production was observed by culture techniques. In addition 3-hydroxy fatty acids, important in biosurfactant production, was observed by FAME analysis. In the all experiments toluene and m+p- xylenes were better growth substrates for both bacteria than the other BTEX compounds. In addition, the test results indicate that the bacteria could contribute to bioremediation of aromatic hydrocarbons (BTEX) pollution increase biodegradation through the action by biosurfactants.

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  • Journal Name: World Journal of Microbiology and Biotechnology

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  • Report No.: WSRC-MS-2006-00352
  • Grant Number: DE-AC09-96SR18500
  • Office of Scientific & Technical Information Report Number: 895964
  • Archival Resource Key: ark:/67531/metadc886849

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Office of Scientific & Technical Information Technical Reports

Reports, articles and other documents harvested from the Office of Scientific and Technical Information.

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  • August 15, 2006

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  • Sept. 22, 2016, 2:13 a.m.

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  • Nov. 2, 2016, 4:14 p.m.

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Brigmon, R; Topher Berry, T; Grazyna A. Plaza, G & jacek Wypych, j. HYDROCARBON-DEGRADING BACTERIA AND SURFACTANT ACTIVITY, article, August 15, 2006; [Aiken, South Carolina]. (digital.library.unt.edu/ark:/67531/metadc886849/: accessed December 12, 2017), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.