Molecular architecture of axonemal microtubule doublets revealedby cryo-electron tomography

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The axoneme, which forms the core of eukaryotic flagella and cilia, is one of the largest macromolecular machines with a structure that is largely conserved from protists to mammals. Microtubule doublets are structural components of axonemes containing a number of proteins besides tubulin, and are usually found in arrays of nine doublets arranged around two singlet microtubules. Coordinated sliding of adjacent doublets, which involves a host of other proteins in the axoneme, produces periodic beating movements of the axoneme. We have obtained a 3D density map of intact microtubule doublets using cryo-electron tomography and image averaging. Our map, with a ... continued below

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Sui, Haixin & Downing, Kenneth H. May 22, 2006.

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The axoneme, which forms the core of eukaryotic flagella and cilia, is one of the largest macromolecular machines with a structure that is largely conserved from protists to mammals. Microtubule doublets are structural components of axonemes containing a number of proteins besides tubulin, and are usually found in arrays of nine doublets arranged around two singlet microtubules. Coordinated sliding of adjacent doublets, which involves a host of other proteins in the axoneme, produces periodic beating movements of the axoneme. We have obtained a 3D density map of intact microtubule doublets using cryo-electron tomography and image averaging. Our map, with a resolution of about 3 nm, provides insights into locations of particular proteins within the doublets and the structural features of the doublets that define their mechanical properties. We identify likely candidates for several of these non-tubulin components of the doublets. This work offers novel insight on how tubulin protofilaments and accessory proteins attach together to form the doublets and provides a structural basis for understanding doublet function in axonemes.

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  • Journal Name: Nature; Journal Volume: 442; Related Information: Journal Publication Date: 07/27/2006

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  • Report No.: LBNL--60216
  • Grant Number: DE-AC02-05CH11231
  • Grant Number: NIHGM46033
  • DOI: 10.1038/nature04816 | External Link
  • Office of Scientific & Technical Information Report Number: 902016
  • Archival Resource Key: ark:/67531/metadc886162

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  • May 22, 2006

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  • Sept. 22, 2016, 2:13 a.m.

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  • Sept. 30, 2016, 12:24 p.m.

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Sui, Haixin & Downing, Kenneth H. Molecular architecture of axonemal microtubule doublets revealedby cryo-electron tomography, article, May 22, 2006; Berkeley, California. (digital.library.unt.edu/ark:/67531/metadc886162/: accessed August 22, 2017), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.