Insights into the O-Acetylation Reaction of Hydroxylated Heterocyclic Amines by Human Arylamine N-Acetyltransferases: A Computational Study

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A computational study was performed to better understand the differences between human arylamine N-acetyltransferase (NAT) 1 and 2. Homology models were constructed from available crystal structures and comparisons of the active site residues 125, 127, and 129 for these two enzymes provide insight into observed substrate differences. The NAT2 model provided a basis for understanding how some of the common mutations may affect the structure of the protein. Molecular dynamics simulations of the human NAT models and the template structure (NAT from Mycobacterium smegmatis) were performed and showed the models to be stable and reasonable. Docking studies of hydroxylated heterocyclic ... continued below

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Lau, E Y; Felton, J S & Lightstone, F C June 6, 2006.

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A computational study was performed to better understand the differences between human arylamine N-acetyltransferase (NAT) 1 and 2. Homology models were constructed from available crystal structures and comparisons of the active site residues 125, 127, and 129 for these two enzymes provide insight into observed substrate differences. The NAT2 model provided a basis for understanding how some of the common mutations may affect the structure of the protein. Molecular dynamics simulations of the human NAT models and the template structure (NAT from Mycobacterium smegmatis) were performed and showed the models to be stable and reasonable. Docking studies of hydroxylated heterocyclic amines in the models of NAT1 and NAT2 probed the differences exhibited by these two proteins with mutagenic agents. The hydroxylated heterocyclic amines were only able to fit into the NAT2 active site, and an alternative binding site by the P-loop was found using our models and will be discussed. Additionally, quantum mechanical calculations were performed to study the O-acetylation reaction of the hydroxylated heterocyclic amines N-OH MeIQx and N-OH PhIP. This study has given us insight into why there are substrate differences among isoenzymes and explains some of the polymorphic activity differences.

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PDF-file: 37 pages; size: 4 Mbytes

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  • Journal Name: Chemical Research in Toxicology, vol. 19, no. 9, September 18, 2006, pp. 1182-1190; Journal Volume: 19; Journal Issue: 9

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  • Report No.: UCRL-JRNL-222764
  • Grant Number: W-7405-ENG-48
  • Office of Scientific & Technical Information Report Number: 907837
  • Archival Resource Key: ark:/67531/metadc884203

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  • June 6, 2006

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  • Sept. 22, 2016, 2:13 a.m.

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  • Nov. 29, 2016, 3:58 p.m.

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Lau, E Y; Felton, J S & Lightstone, F C. Insights into the O-Acetylation Reaction of Hydroxylated Heterocyclic Amines by Human Arylamine N-Acetyltransferases: A Computational Study, article, June 6, 2006; Livermore, California. (digital.library.unt.edu/ark:/67531/metadc884203/: accessed July 17, 2018), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.