Molecular Mechanism of Uranium Reduction by Clostridia and its Manipulation

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Description

This research addresses the need for detailed studies of the enzymatic mechanisms for reduction of radionuclides and/or metals by fermentative microorganisms. The overall objective of this research is to elucidate systematically the molecular mechanisms involved in the reduction of uranium by Clostridia. We propose to (1) determine the role of hydrogenases in uranium reduction, (22) purify the enzymes involved in uranium reduction, (3) determine the mechanisms of reduction, e.g., one or two electron transfer reactions, and (4) elucidate the genetic control of the enzymes and cellular factors involved in uranium reduction. This is a collaborative study between BNL and Stanford ... continued below

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Francis, A. J.; W. Gao, D. Chidambaram & Dodge, C.J. June 1, 2006.

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Description

This research addresses the need for detailed studies of the enzymatic mechanisms for reduction of radionuclides and/or metals by fermentative microorganisms. The overall objective of this research is to elucidate systematically the molecular mechanisms involved in the reduction of uranium by Clostridia. We propose to (1) determine the role of hydrogenases in uranium reduction, (22) purify the enzymes involved in uranium reduction, (3) determine the mechanisms of reduction, e.g., one or two electron transfer reactions, and (4) elucidate the genetic control of the enzymes and cellular factors involved in uranium reduction. This is a collaborative study between BNL and Stanford University involving expertise in biomolecular science, biochemistry, microbiology, and electrochemistry.

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  • Report No.: ERSD-1026860-2006
  • Grant Number: None
  • DOI: 10.2172/896246 | External Link
  • Office of Scientific & Technical Information Report Number: 896246
  • Archival Resource Key: ark:/67531/metadc883933

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  • June 1, 2006

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  • Sept. 22, 2016, 2:13 a.m.

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  • Nov. 4, 2016, 4:19 p.m.

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Francis, A. J.; W. Gao, D. Chidambaram & Dodge, C.J. Molecular Mechanism of Uranium Reduction by Clostridia and its Manipulation, report, June 1, 2006; Upton, New York. (digital.library.unt.edu/ark:/67531/metadc883933/: accessed August 20, 2017), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.