Towards a More Complete Picture: Dissimilatory Metal Reduction by Anaeromyxobacter Species

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We investigate the physiological requirements of available Anaeromyxobacter isolates, and assess their distribution and abundance in the environment, including DOE sites. The performers on this project include Frank Loeffler (PI), Robert Sanford (Co-PI), Qingzhong Wu (postdoc), Sara Henry (graduate student) and Cornell Gayle (undergraduate student). Year-1 efforts focused on method and tool development to address the research objectives. First, we compared different analytical assays (based on fluorescent light emission and calorimetric methods) to quantify U(VI) in cultures of Anaeromyxobacter dehalogenans strain 2CP-C. The assays were optimized to reflect specific culture conditions, and we found that a laser-excited spectrofluorescence assay provided ... continued below

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Loeffler, Frank E. June 1, 2004.

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Description

We investigate the physiological requirements of available Anaeromyxobacter isolates, and assess their distribution and abundance in the environment, including DOE sites. The performers on this project include Frank Loeffler (PI), Robert Sanford (Co-PI), Qingzhong Wu (postdoc), Sara Henry (graduate student) and Cornell Gayle (undergraduate student). Year-1 efforts focused on method and tool development to address the research objectives. First, we compared different analytical assays (based on fluorescent light emission and calorimetric methods) to quantify U(VI) in cultures of Anaeromyxobacter dehalogenans strain 2CP-C. The assays were optimized to reflect specific culture conditions, and we found that a laser-excited spectrofluorescence assay provided reproducible and accurate information on the amount of U(VI) reduced in bacterial cultures. To demonstrate the ability of Anaeromyxobacter dehalogenans strain 2CP-C to reduce U(VI), washed suspensions of fumarate-grown cells were prepared. These experiments confirmed that the rapid reduction of U(VI) to U(IV) depended on the presence of live cells, and no U(VI) reduction occurred in cell-free controls. Additional experiments explored the ability of three different Anaeromyxobacter strains to grow with the mineral hematite, an insoluble form of ferric iron, as electron acceptor. All strain grew equally well with soluble ferric iron (provided as ferric citrate) but distinct differences were observed between strains when grown with hematite. All strains tested shared a 16S rRNA gene similarity of >99.5%, suggesting that closely related strains may differ in their ability to access insoluble forms of ferric iron.

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  • Report No.: NABIR-1022485-2004
  • Grant Number: None
  • DOI: 10.2172/893685 | External Link
  • Office of Scientific & Technical Information Report Number: 893685
  • Archival Resource Key: ark:/67531/metadc883495

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  • June 1, 2004

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  • Sept. 21, 2016, 2:29 a.m.

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  • Nov. 4, 2016, 5:45 p.m.

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Loeffler, Frank E. Towards a More Complete Picture: Dissimilatory Metal Reduction by Anaeromyxobacter Species, report, June 1, 2004; United States. (digital.library.unt.edu/ark:/67531/metadc883495/: accessed September 25, 2017), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.