Identification of Metal Reductases using Proteomic Analysis

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Central to the NABIR goal to develop the scientific basis for in situ remediation of radioactive contaminants is the fundamental understanding of microorganisms with dissimilatory metal reducing activity. In order to effectively exploit these bacteria, it is necessary to know which enzymes and pathways are involved. Additionally, it would be advantageous to understand the similarities and differences of these pathways across different bacteria for effective deployment in bioremediation, as well as to identify new microbes capable of such activities. Most approaches to identify these enzymes or enzyme complexes rely on biochemical purification to homogeneity with subsequent Nterminal sequencing of digested ... continued below

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Lipton, Mary June 1, 2006.

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Description

Central to the NABIR goal to develop the scientific basis for in situ remediation of radioactive contaminants is the fundamental understanding of microorganisms with dissimilatory metal reducing activity. In order to effectively exploit these bacteria, it is necessary to know which enzymes and pathways are involved. Additionally, it would be advantageous to understand the similarities and differences of these pathways across different bacteria for effective deployment in bioremediation, as well as to identify new microbes capable of such activities. Most approaches to identify these enzymes or enzyme complexes rely on biochemical purification to homogeneity with subsequent Nterminal sequencing of digested peptides. However, loss of activity before achieving purity often necessitates repetition of the entire process. Newly developed proteomics capabilities at PNNL allow for the identification of many proteins from a single sample through mass spectrometry analysis.

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  • Report No.: ERSD-1022403-2006
  • Grant Number: None
  • DOI: 10.2172/896018 | External Link
  • Office of Scientific & Technical Information Report Number: 896018
  • Archival Resource Key: ark:/67531/metadc880802

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Office of Scientific & Technical Information Technical Reports

Reports, articles and other documents harvested from the Office of Scientific and Technical Information.

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  • June 1, 2006

Added to The UNT Digital Library

  • Sept. 22, 2016, 2:13 a.m.

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  • Nov. 4, 2016, 6:35 p.m.

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Lipton, Mary. Identification of Metal Reductases using Proteomic Analysis, report, June 1, 2006; Richland, Washington. (digital.library.unt.edu/ark:/67531/metadc880802/: accessed November 20, 2017), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.