Genes for Uranium Bioremediation in the Anaerobic Sulfate-Reducing Bacteria

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Objective A: Electron transfer components necessary for uranium reduction. Objective B: Possible FNR-analog in the sulfate-reducing bacteria. Attempts to isolate FNR or FIKJ analogs from Desuflovibrio through the design of degenerate primers for amplification of portions of the genes has not been successful. In contrast, several amplicons have been generated for the genes encoding the regulators of two-component signal sequences. Since several global regulators fall into this class, we are attempting to obtain sufficient sequence information to indicate what metabolic pathways are affected by the regulators. Cloning and sequencing of two such amplicons has revealed that bona fide two-component regulators ... continued below

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Wall, Judy D. June 1, 1999.

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Objective A: Electron transfer components necessary for uranium reduction. Objective B: Possible FNR-analog in the sulfate-reducing bacteria. Attempts to isolate FNR or FIKJ analogs from Desuflovibrio through the design of degenerate primers for amplification of portions of the genes has not been successful. In contrast, several amplicons have been generated for the genes encoding the regulators of two-component signal sequences. Since several global regulators fall into this class, we are attempting to obtain sufficient sequence information to indicate what metabolic pathways are affected by the regulators. Cloning and sequencing of two such amplicons has revealed that bona fide two-component regulators are present in Desulfovibrio.

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  • Report No.: NABIR-1010098-1999
  • Grant Number: None
  • DOI: 10.2172/893937 | External Link
  • Office of Scientific & Technical Information Report Number: 893937
  • Archival Resource Key: ark:/67531/metadc880638

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  • June 1, 1999

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  • Sept. 22, 2016, 2:13 a.m.

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  • Oct. 31, 2016, 9:01 p.m.

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Wall, Judy D. Genes for Uranium Bioremediation in the Anaerobic Sulfate-Reducing Bacteria, report, June 1, 1999; United States. (digital.library.unt.edu/ark:/67531/metadc880638/: accessed September 25, 2017), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.