Advanced microscopy :time-resolved multi-spectral imaging of single biomolecules.

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Description

Over the past few years we have developed the ability to acquire images through a confocal microscope that contain, for each pixel, the simultaneous fluorescence lifetime and spectra of multiple fluorophores within that pixel. We have demonstrated that our system has the sensitivity to make these measurements on single molecules. The spectra and lifetimes of fluorophores bound to complex molecules contain a wealth of information on the conformational dynamics and local chemical environments of the molecules. However, the detailed record of spectral and temporal information our system provides from fluorophores in single molecules has not been previously available. Therefore, we ... continued below

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21 p.

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Hayden, Carl C.; Chandler, David W.; Gradinaru, Claudiu C. & Luong, A. Khai December 1, 2005.

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Description

Over the past few years we have developed the ability to acquire images through a confocal microscope that contain, for each pixel, the simultaneous fluorescence lifetime and spectra of multiple fluorophores within that pixel. We have demonstrated that our system has the sensitivity to make these measurements on single molecules. The spectra and lifetimes of fluorophores bound to complex molecules contain a wealth of information on the conformational dynamics and local chemical environments of the molecules. However, the detailed record of spectral and temporal information our system provides from fluorophores in single molecules has not been previously available. Therefore, we have studied several fluorophores and simple fluorophore-molecule systems that are representative of the use of fluorophores in biological systems. Experiments include studies of a simple fluorescence resonance energy transfer (FRET) system, green fluorescent probe variants and quantum dots. This work is intended to provide a basis for understanding how fluorophores report on the chemistry of more complex biological molecules.

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21 p.

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  • Report No.: SAND2005-7061
  • Grant Number: AC04-94AL85000
  • DOI: 10.2172/877146 | External Link
  • Office of Scientific & Technical Information Report Number: 877146
  • Archival Resource Key: ark:/67531/metadc880118

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Office of Scientific & Technical Information Technical Reports

Reports, articles and other documents harvested from the Office of Scientific and Technical Information.

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  • December 1, 2005

Added to The UNT Digital Library

  • Sept. 21, 2016, 2:29 a.m.

Description Last Updated

  • Nov. 22, 2016, 3:57 p.m.

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Hayden, Carl C.; Chandler, David W.; Gradinaru, Claudiu C. & Luong, A. Khai. Advanced microscopy :time-resolved multi-spectral imaging of single biomolecules., report, December 1, 2005; United States. (digital.library.unt.edu/ark:/67531/metadc880118/: accessed October 20, 2017), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.