Whole-genome analyses of the speciation events in the pathogenic Brucellae Metadata

Title

• Main Title Whole-genome analyses of the speciation events in the pathogenic Brucellae

Creator

• Author: Chain, P,
  Creator Type: Personal
• Author: Comerci, D.
  Creator Type: Personal
• Author: Tolmasky, M.
  Creator Type: Personal
• Author: Larimer, F.
  Creator Type: Personal
• Author: Malfatti, S.
  Creator Type: Personal
• Author: Vergez, L.
  Creator Type: Personal
• Author: Aguero, F.
  Creator Type: Personal
• Author: Land, M.
  Creator Type: Personal
• Author: Ugalde, R.
  Creator Type: Personal
• Author: Garcia, E.
  Creator Type: Personal

Contributor

• Sponsor: United States. Department of Energy.
  Contributor Type: Organization

Publisher

• Name: Lawrence Livermore National Laboratory
  Place of Publication: Livermore, California
  Additional Info: Lawrence Livermore National Laboratory (LLNL), Livermore, CA

Date

• Creation: 2005-07-14

Language

• English

Description

• Content Description: Despite their high DNA identity and a proposal to group classical Brucella species as biovars of B. melitensis, the commonly recognized Brucella species can be distinguished by distinct biochemical and fatty acid characters as well as by a marked host range (e.g. B. suis for swine, B. melitensis for sheep and goats, B. abortus for cattle). Here we present the genome of B. abortus 2308, the virulent prototype biovar 1 strain, and its comparison to the two other human pathogenic Brucellae species and to the B. abortus field isolate 9-941. The global distribution of pseudogenes, deletions and insertions support previous indications that B. abortus and B. melitensis share a common ancestor that diverged from B. suis. With the exception of a dozen genes, the genetic complement of both B. abortus strains is identical, whereas the three species differ in gene content and pseudogenes. The pattern of species-specific gene inactivations affecting transcriptional regulators and outer membrane proteins suggest that these inactivations may play an important role in the establishment of host-specificity and may have been a primary driver of speciation in the Brucellae. Despite being non-motile, the Brucellae contain flagellum gene clusters and display species-specific flagellar gene inactivations, which lead to the putative generation of different versions of flagellum-derived structures, and may contribute to differences in host-specificity and virulence. Metabolic changes such as the lack of complete metabolic pathways for the synthesis of numerous compounds (e.g. glycogen, biotin, NAD, and choline) are consistent with adaptation of Brucellae to an intracellular lifestyle.
• Physical Description: PDF-file: 37 pages; size: 1.1 Mbytes

Subject

• Keyword: Dna
• Keyword: Genetics
• Keyword: Glycogen
• Keyword: Genes
• Keyword: Synthesis
• Keyword: Sheep
• Keyword: Swine
• STI Subject Categories: 59 Basic Biological Sciences
• Keyword: Brucella
• Keyword: Membrane Proteins
• Keyword: Biotin
• Keyword: Strains
• Keyword: Cattle
• Keyword: Virulence
• Keyword: Choline
• Keyword: Biological Pathways
• Keyword: Carboxylic Acids
• Keyword: Goats

Source

• Journal Name: Infection and Immunity; Journal Volume: 73; Journal Issue: 12

Collection

• Name: Office of Scientific & Technical Information Technical Reports
  Code: OSTI

Institution

• Name: UNT Libraries Government Documents Department
  Code: UNTGD

Resource Type

• Article

Format

• Text

Identifier

• Report No.: UCRL-JRNL-213629
• Grant Number: W-7405-ENG-48
• Office of Scientific & Technical Information Report Number: 878626
• Archival Resource Key: ark:/67531/metadc878026