A Simple Approach for Obtaining High Resolution, High Sensitivity ¹H NMR Metabolite Spectra of Biofluids with Limited Mass Supply

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A simple approach is reported that yields high resolution, high sensitivity ¹H NMR spectra of biofluids with limited mass supply. This is achieved by spinning a capillary sample tube containing a biofluid at the magic angle at a frequency of about 80Hz. A 2D pulse sequence called ¹H PASS is then used to produce a high-resolution ¹H NMR spectrum that is free from magnetic susceptibility induced line broadening. With this new approach a high resolution ¹H NMR spectrum of biofluids with a volume less than 1.0 µl can be easily achieved at a magnetic field strength as low as 7.05T. ... continued below

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Hu, Jian Zhi; Rommereim, Donald N.; Wind, Robert A.; Minard, Kevin R. & Sears, Jesse A. November 1, 2006.

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A simple approach is reported that yields high resolution, high sensitivity ¹H NMR spectra of biofluids with limited mass supply. This is achieved by spinning a capillary sample tube containing a biofluid at the magic angle at a frequency of about 80Hz. A 2D pulse sequence called ¹H PASS is then used to produce a high-resolution ¹H NMR spectrum that is free from magnetic susceptibility induced line broadening. With this new approach a high resolution ¹H NMR spectrum of biofluids with a volume less than 1.0 µl can be easily achieved at a magnetic field strength as low as 7.05T. Furthermore, the methodology facilitates easy sample handling, i.e., the samples can be directly collected into inexpensive and disposable capillary tubes at the site of collection and subsequently used for NMR measurements. In addition, slow magic angle spinning improves magnetic field shimming and is especially suitable for high throughput investigations. In this paper first results are shown obtained in a magnetic field of 7.05T on urine samples collected from mice using a modified commercial NMR probe.

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  • Proceedings of the 2006 International Conference on Bioinformatics and Computational Biology (BIOCOMP '06), 360-366

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  • Report No.: PNNL-SA-48823
  • Grant Number: AC05-76RL01830
  • Office of Scientific & Technical Information Report Number: 899490
  • Archival Resource Key: ark:/67531/metadc877810

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Reports, articles and other documents harvested from the Office of Scientific and Technical Information.

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  • November 1, 2006

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  • Sept. 22, 2016, 2:13 a.m.

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  • Dec. 7, 2016, 9:13 p.m.

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Hu, Jian Zhi; Rommereim, Donald N.; Wind, Robert A.; Minard, Kevin R. & Sears, Jesse A. A Simple Approach for Obtaining High Resolution, High Sensitivity ¹H NMR Metabolite Spectra of Biofluids with Limited Mass Supply, article, November 1, 2006; (digital.library.unt.edu/ark:/67531/metadc877810/: accessed December 17, 2017), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.