Feasibility of Single Molecule DNA Sequencing using Surface-Enhanced Raman Scattering

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We have used a combined theoretical and experimental approach in order to assess the feasibility of using surface-enhanced Raman scattering (SERS) for DNA sequencing at the single molecule level. We have developed a numerical tool capable of calculating the E-field and resulting SERS enhancement factors for metallic structures of arbitrary size and shape. Measurements of the additional SERS enhancement by combining SERS with coherent antistokes Raman scattering (CARS) show that only modest increases in the signal are achievable due to thermal damage at higher laser powers. Finally, measurements of the SERS enhancement from nanoparticles coated with an insulating layer show ... continued below

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Talley, C E; Reboredo, F; Chan, J & Lane, S M February 3, 2006.

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We have used a combined theoretical and experimental approach in order to assess the feasibility of using surface-enhanced Raman scattering (SERS) for DNA sequencing at the single molecule level. We have developed a numerical tool capable of calculating the E-field and resulting SERS enhancement factors for metallic structures of arbitrary size and shape. Measurements of the additional SERS enhancement by combining SERS with coherent antistokes Raman scattering (CARS) show that only modest increases in the signal are achievable due to thermal damage at higher laser powers. Finally, measurements of the SERS enhancement from nanoparticles coated with an insulating layer show that the SERS enhancement is decreased by as much as two orders of magnitude when the molecule is not in contact with the metal surface.

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PDF-file: 29 pages; size: 1 Mbytes

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  • Report No.: UCRL-TR-218737
  • Grant Number: W-7405-ENG-48
  • DOI: 10.2172/899105 | External Link
  • Office of Scientific & Technical Information Report Number: 899105
  • Archival Resource Key: ark:/67531/metadc877688

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Office of Scientific & Technical Information Technical Reports

Reports, articles and other documents harvested from the Office of Scientific and Technical Information.

Office of Scientific and Technical Information (OSTI) is the Department of Energy (DOE) office that collects, preserves, and disseminates DOE-sponsored research and development (R&D) results that are the outcomes of R&D projects or other funded activities at DOE labs and facilities nationwide and grantees at universities and other institutions.

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  • February 3, 2006

Added to The UNT Digital Library

  • Sept. 22, 2016, 2:13 a.m.

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  • Nov. 28, 2016, 3:21 p.m.

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Talley, C E; Reboredo, F; Chan, J & Lane, S M. Feasibility of Single Molecule DNA Sequencing using Surface-Enhanced Raman Scattering, report, February 3, 2006; Livermore, California. (digital.library.unt.edu/ark:/67531/metadc877688/: accessed May 21, 2018), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.