Raman Spectroscopic Analysis of Biochemical Changes in Individual Triglyceride-Rich Lipoproteins in the Pre- and Postprandial State Metadata

Metadata describes a digital item, providing (if known) such information as creator, publisher, contents, size, relationship to other resources, and more. Metadata may also contain "preservation" components that help us to maintain the integrity of digital files over time.

Title

  • Main Title Raman Spectroscopic Analysis of Biochemical Changes in Individual Triglyceride-Rich Lipoproteins in the Pre- and Postprandial State

Creator

  • Author: Chan, J
    Creator Type: Personal
  • Author: Motton, D
    Creator Type: Personal
  • Author: Rutledge, J
    Creator Type: Personal
  • Author: Keim, N
    Creator Type: Personal
  • Author: Huser, T
    Creator Type: Personal

Contributor

  • Sponsor: United States. Department of Energy.
    Contributor Type: Organization

Publisher

  • Name: Lawrence Livermore National Laboratory
    Place of Publication: Livermore, California
    Additional Info: Lawrence Livermore National Laboratory (LLNL), Livermore, CA

Date

  • Creation: 2004-09-13

Language

  • English

Description

  • Content Description: Individual triglyceride-rich lipoprotein (TGRL) particles derived from human volunteers are non-destructively analyzed by laser tweezers Raman microspectroscopy and information on their composition and distribution is obtained. The Raman signature of single optically trapped very low-density lipoproteins (VLDL), a subclass of TGRL, which play an important role in cardiovascular disease, exhibits distinct peaks associated with molecular vibrations of fatty acids, proteins, lipids, and structural rearrangements of lipids. Our analysis of pre- and postprandial VLDL exhibits the signature of biochemical changes in individual lipoprotein particles following the consumption of meals. Interaction of VLDL with endothelium leads to the breakdown of complex triacylglycerols and the formation of a highly ordered core of free saturated fatty acids in the particle. A particle distribution analysis reveals trends in the degree to which this process has occurred in particles at different times during the postprandial period. Differences in particle distributions based on the different ratios of polyunsaturated to saturated fats in the consumed meals are also easily discerned. Individual lipoprotein particles hydrolyzed in-vitro through addition of lipoprotein lipase (LpL) exhibit strikingly similar changes in their Raman spectra. These results demonstrate the feasibility of monitoring the dynamics of lipid metabolism of individual TGRL particles as they interact with LpL in the endothelial cell wall using Raman spectroscopy.
  • Physical Description: PDF-file: 31 pages; size: 1.4 Mbytes

Subject

  • Keyword: Cardiovascular Diseases
  • Keyword: Breakdown
  • Keyword: In Vitro
  • Keyword: Monitoring
  • Keyword: Fats
  • Keyword: Endothelium
  • Keyword: Raman Spectra
  • STI Subject Categories: 59 Basic Biological Sciences
  • Keyword: Proteins
  • STI Subject Categories: 42 Engineering
  • STI Subject Categories: 71 Classical And Quantum Mechanics, General Physics
  • Keyword: Raman Spectroscopy
  • Keyword: Lipases
  • Keyword: Lipids
  • Keyword: Lasers
  • Keyword: Distribution
  • Keyword: Cell Wall
  • STI Subject Categories: 60 Applied Life Sciences
  • Keyword: Metabolism
  • Keyword: Carboxylic Acids
  • Keyword: Lipoproteins

Source

  • Journal Name: Analytical Chemistry; Journal Volume: 77; Journal Issue: 18

Collection

  • Name: Office of Scientific & Technical Information Technical Reports
    Code: OSTI

Institution

  • Name: UNT Libraries Government Documents Department
    Code: UNTGD

Resource Type

  • Article

Format

  • Text

Identifier

  • Report No.: UCRL-JRNL-206592
  • Grant Number: W-7405-ENG-48
  • Office of Scientific & Technical Information Report Number: 875661
  • Archival Resource Key: ark:/67531/metadc877455