Micro-Raman spectroscopy Detects Individual Neoplastic and Normal Hematopoietic Cells

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Current methods for identifying neoplastic cells and discerning them from their normal counterparts are often non-specific, slow, biologically perturbing, or a combination, thereof. Here, we show that single-cell micro-Raman spectroscopy averts these shortcomings and can be used to discriminate between unfixed normal human lymphocytes and transformed Jurkat and Raji lymphocyte cell lines based on their biomolecular Raman signatures. We demonstrate that single-cell Raman spectra provide a highly reproducible biomolecular fingerprint of each cell type. Characteristic peaks, mostly due to different DNA and protein concentrations, allow for discerning normal lymphocytes from transformed lymphocytes with high confidence (p << 0.05). Spectra are ... continued below

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PDF-file: 24 pages; size: 0.7 Mbytes

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Chan, J W; Taylor, D; Zwerdling, T; Lane, S M; Ihara, K & Huser, T January 18, 2005.

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Current methods for identifying neoplastic cells and discerning them from their normal counterparts are often non-specific, slow, biologically perturbing, or a combination, thereof. Here, we show that single-cell micro-Raman spectroscopy averts these shortcomings and can be used to discriminate between unfixed normal human lymphocytes and transformed Jurkat and Raji lymphocyte cell lines based on their biomolecular Raman signatures. We demonstrate that single-cell Raman spectra provide a highly reproducible biomolecular fingerprint of each cell type. Characteristic peaks, mostly due to different DNA and protein concentrations, allow for discerning normal lymphocytes from transformed lymphocytes with high confidence (p << 0.05). Spectra are also compared and analyzed by principal component analysis (PCA) to demonstrate that normal and transformed cells form distinct clusters that can be defined using just two principal components. The method is shown to have a sensitivity of 98.3% for cancer detection, with 97.2% of the cells being correctly classified as belonging to the normal or transformed type. These results demonstrate the potential application of confocal micro-Raman spectroscopy as a clinical tool for single cell cancer detection based on intrinsic biomolecular signatures, therefore eliminating the need for exogenous fluorescent labeling.

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PDF-file: 24 pages; size: 0.7 Mbytes

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  • Journal Name: Biophysical Journal; Journal Volume: 90

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  • Report No.: UCRL-JRNL-209131
  • Grant Number: W-7405-ENG-48
  • Office of Scientific & Technical Information Report Number: 875963
  • Archival Resource Key: ark:/67531/metadc876654

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  • January 18, 2005

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  • Sept. 21, 2016, 2:29 a.m.

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  • Nov. 29, 2016, 8:09 p.m.

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Chan, J W; Taylor, D; Zwerdling, T; Lane, S M; Ihara, K & Huser, T. Micro-Raman spectroscopy Detects Individual Neoplastic and Normal Hematopoietic Cells, article, January 18, 2005; Livermore, California. (digital.library.unt.edu/ark:/67531/metadc876654/: accessed September 21, 2018), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.