A Link between Meiotic Prophase Progression and CrossoverControl

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During meiosis, most organisms ensure that homologous chromosomes undergo at least one exchange of DNA, or crossover, to link chromosomes together and accomplish proper segregation. How each chromosome receives a minimum of one crossover is unknown. During early meiosis in Caenorhabditis elegans and many other species, chromosomes adopt a polarized organization within the nucleus, which normally disappears upon completion of homolog synapsis. Mutations that impair synapsis even between a single pair of chromosomes in C. elegans delay this nuclear reorganization. We quantified this delay by developing a classification scheme for discrete stages of meiosis. Immunofluorescence localization of RAD-51 protein revealed ... continued below

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Carlton, Peter M.; Farruggio, Alfonso P. & Dernburg, Abby F. July 6, 2005.

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During meiosis, most organisms ensure that homologous chromosomes undergo at least one exchange of DNA, or crossover, to link chromosomes together and accomplish proper segregation. How each chromosome receives a minimum of one crossover is unknown. During early meiosis in Caenorhabditis elegans and many other species, chromosomes adopt a polarized organization within the nucleus, which normally disappears upon completion of homolog synapsis. Mutations that impair synapsis even between a single pair of chromosomes in C. elegans delay this nuclear reorganization. We quantified this delay by developing a classification scheme for discrete stages of meiosis. Immunofluorescence localization of RAD-51 protein revealed that delayed meiotic cells also contained persistent recombination intermediates. Through genetic analysis, we found that this cytological delay in meiotic progression requires double-strand breaks and the function of the crossover-promoting heteroduplex HIM-14 (Msh4) and MSH-5. Failure of X chromosome synapsis also resulted in impaired crossover control on autosomes, which may result from greater numbers and persistence of recombination intermediates in the delayed nuclei. We conclude that maturation of recombination events on chromosomes promotes meiotic progression, and is coupled to the regulation of crossover number and placement. Our results have broad implications for the interpretation of meiotic mutants, as we have shown that asynapsis of a single chromosome pair can exert global effects on meiotic progression and recombination frequency.

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  • Journal Name: Public Library of Science (PLoS) Genetics; Journal Volume: 2; Journal Issue: 2; Related Information: Journal Publication Date: 02/2006

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  • Report No.: LBNL--58822
  • Grant Number: DE-AC02-05CH11231
  • Grant Number: NIH:R1GM65591A
  • Office of Scientific & Technical Information Report Number: 890634
  • Archival Resource Key: ark:/67531/metadc874614

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  • July 6, 2005

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  • Sept. 21, 2016, 2:29 a.m.

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  • Dec. 9, 2016, 10:17 p.m.

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Carlton, Peter M.; Farruggio, Alfonso P. & Dernburg, Abby F. A Link between Meiotic Prophase Progression and CrossoverControl, article, July 6, 2005; Berkeley, California. (digital.library.unt.edu/ark:/67531/metadc874614/: accessed August 23, 2017), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.