Optimization of Xenon Biosensors for Detection of ProteinInteractions

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Hyperpolarized 129Xe NMR can detect the presence of specific low-concentration biomolecular analytes by means of the xenon biosensor, which consists of a water-soluble, targeted cryptophane-A cage that encapsulates xenon. In this work we use the prototypical biotinylated xenon biosensor to determine the relationship between the molecular composition of the xenon biosensor and the characteristics of protein-bound resonances. The effects of diastereomer overlap, dipole-dipole coupling, chemical shift anisotropy, xenon exchange, and biosensor conformational exchange on protein-bound biosensor signal were assessed. It was found that optimal protein-bound biosensor signal can be obtained by minimizing the number of biosensor diastereomers and using a ... continued below

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Lowery, Thomas J.; Garcia, Sandra; Chavez, Lana; Ruiz, E.Janette; Wu, Tom; Brotin, Thierry et al. August 3, 2005.

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Hyperpolarized 129Xe NMR can detect the presence of specific low-concentration biomolecular analytes by means of the xenon biosensor, which consists of a water-soluble, targeted cryptophane-A cage that encapsulates xenon. In this work we use the prototypical biotinylated xenon biosensor to determine the relationship between the molecular composition of the xenon biosensor and the characteristics of protein-bound resonances. The effects of diastereomer overlap, dipole-dipole coupling, chemical shift anisotropy, xenon exchange, and biosensor conformational exchange on protein-bound biosensor signal were assessed. It was found that optimal protein-bound biosensor signal can be obtained by minimizing the number of biosensor diastereomers and using a flexible linker of appropriate length. Both the linewidth and sensitivity of chemical shift to protein binding of the xenon biosensor were found to be inversely proportional to linker length.

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  • Journal Name: ChemBioChem; Journal Volume: 7; Journal Issue: 1; Related Information: Journal Publication Date: 01/2006

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  • Report No.: LBNL--58607
  • Grant Number: DE-AC02-05CH11231
  • Office of Scientific & Technical Information Report Number: 878709
  • Archival Resource Key: ark:/67531/metadc874103

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  • August 3, 2005

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  • Sept. 21, 2016, 2:29 a.m.

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  • Sept. 30, 2016, 12:29 p.m.

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Lowery, Thomas J.; Garcia, Sandra; Chavez, Lana; Ruiz, E.Janette; Wu, Tom; Brotin, Thierry et al. Optimization of Xenon Biosensors for Detection of ProteinInteractions, article, August 3, 2005; Berkeley, California. (digital.library.unt.edu/ark:/67531/metadc874103/: accessed August 22, 2017), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.