Developing a Biosensor for Estrogens in Water Samples: Study ofthe Real-time Response of Live Cells of the Estrogen-sensitive YeastStrain RMY/ER-ERE using Fluorescence Microscopy

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Using a fluorescein di-{beta}-d-galactopyranoside (FDG) substrate we show that in live cells of an estrogen-sensitive yeast strain RMY/ER-ERE with human estrogen receptor (ER{alpha}) gene and the lacZ gene which encodes {beta}-galactosidase, the uptake of 17{beta}-estradiol (E2) and the subsequent production of {beta}-galactosidase enzyme occur quite rapidly, with maximal enzyme-catalyzed product formation evident after about 30 min of exposure to E2. This finding which agrees with the well-known rates of enzyme-catalyzed reactions could have implications for shortening the duration of environmental sample screening and monitoring regimes using yeast-based estrogen assays, and the development of biosensors for environmental estrogens to complement quantification ... continued below

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Wozei, E.; Hermanowicz, S.W. & Holman, H-Y.N. January 1, 2006.

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Using a fluorescein di-{beta}-d-galactopyranoside (FDG) substrate we show that in live cells of an estrogen-sensitive yeast strain RMY/ER-ERE with human estrogen receptor (ER{alpha}) gene and the lacZ gene which encodes {beta}-galactosidase, the uptake of 17{beta}-estradiol (E2) and the subsequent production of {beta}-galactosidase enzyme occur quite rapidly, with maximal enzyme-catalyzed product formation evident after about 30 min of exposure to E2. This finding which agrees with the well-known rates of enzyme-catalyzed reactions could have implications for shortening the duration of environmental sample screening and monitoring regimes using yeast-based estrogen assays, and the development of biosensors for environmental estrogens to complement quantification methods.

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  • Journal Name: Biosensors and Bioelectronics; Journal Volume: 21; Journal Issue: 8; Related Information: Journal Publication Date: 02/15/2006

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  • Report No.: LBNL--58194
  • Grant Number: DE-AC02-05CH11231
  • DOI: 10.1016/j.bios.2005.07.021 | External Link
  • Office of Scientific & Technical Information Report Number: 882168
  • Archival Resource Key: ark:/67531/metadc873728

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  • January 1, 2006

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  • Sept. 21, 2016, 2:29 a.m.

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  • Sept. 29, 2016, 2:26 p.m.

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Wozei, E.; Hermanowicz, S.W. & Holman, H-Y.N. Developing a Biosensor for Estrogens in Water Samples: Study ofthe Real-time Response of Live Cells of the Estrogen-sensitive YeastStrain RMY/ER-ERE using Fluorescence Microscopy, article, January 1, 2006; Berkeley, California. (digital.library.unt.edu/ark:/67531/metadc873728/: accessed September 21, 2017), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.