ACCUMULATION AND RETENTION OF TRITIUM (TRITIATED WATER) IN EMBRYONIC AND LARVAL FISH, AND RADIATION EFFECT. Page: 4 of 23
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Eggs of rainbow trout, Salmo gairdneri, were spawned artificially
in the laboratory and exposed to 0.01, 0.1, 1.0 and 10.0 'U/ml tritium-H3
(biological grade) contaminated spring water. Rearing throughout
embryogenesis, 25 days at 10.5 + .5* C, was facilitated within a"
recirculating drip incubation system. Water and embryos were sampled
at intervals to ascertain the time necessary to reach equilibrium.
After 20 days exposure, selected lots of embryos were removed to
inactive free-flowing water to study retention and turnover components.
Combustion of samples for liquid scintillation counting was accomplished
using Model 300 Tri-Carb Sample Oxidizer. Distinction between volatile'
' or "body-water" tritium-H3 and non-volatile or "bound". tritium-H3 was
A rapid uptake of tritium-H3 during the first 1 to 2 days was
indicated for all three concentrations,' followed during the ensuing
27 to 28 days of exposure by maintenance of equilibrium levels.' The
"bound" tritium-H fraction accounted for approximately.20 percent of the
total label in each egg, a level not exceeded as measured over the duration
of exposure. Both Volatile and "bound" fractions, after transfer to
"free-flowing water, elute rapidly with estimated half-times of "1.0 and
t2 hours, respectively. A slower component of the volatile fractions
persists for "'17 days, as determined in hatched fry. The slow component
of the "bound". fraction, for comparison, persists at approximately 0.6
percent of the initial "bound" equilibrium level at 17 days. Even after
six months, in juveniles, a trace but measurable. level of this component
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Strand, J. A.; Templeton, W. L. & Tangen, E. G. ACCUMULATION AND RETENTION OF TRITIUM (TRITIATED WATER) IN EMBRYONIC AND LARVAL FISH, AND RADIATION EFFECT., report, October 31, 1971; Richland, Washington. (https://digital.library.unt.edu/ark:/67531/metadc871610/m1/4/: accessed March 25, 2019), University of North Texas Libraries, Digital Library, https://digital.library.unt.edu; crediting UNT Libraries Government Documents Department.