Influence of cell dissociation procedures on the tumorigenicity of Simian Virus 40 transformed fibroblasts

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Mouse fibroblasts transformed by Simian Virus 40 (SV40) were examined for tumor forming ability in syngeneic BALB/c mice following dissociation from tissue culture dishes by two procedures. A significantly greater in vivo proliferative capacity was observed for cells dissociated by the tryspin-EDTA procedure, with the injected cell dose for tumor production in 50 percent of recipient mice (the TPD$sub 50$) being 16-fold lower than the TPD$sub 50$ for cells dissociated by the EDTA procedure. Host immunosuppression with 300 rad whole-body $gamma$ irradiation led to a significant 7-fold decrease in the TPD$sub 50$ for cells dissociated by the EDTA procedure, while ... continued below

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15 p.

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Tenforde, T.S.; Risius, J.; Beckmann, A.; Tobias, C.A. & Gurney, E. November 1, 1975.

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Mouse fibroblasts transformed by Simian Virus 40 (SV40) were examined for tumor forming ability in syngeneic BALB/c mice following dissociation from tissue culture dishes by two procedures. A significantly greater in vivo proliferative capacity was observed for cells dissociated by the tryspin-EDTA procedure, with the injected cell dose for tumor production in 50 percent of recipient mice (the TPD$sub 50$) being 16-fold lower than the TPD$sub 50$ for cells dissociated by the EDTA procedure. Host immunosuppression with 300 rad whole-body $gamma$ irradiation led to a significant 7-fold decrease in the TPD$sub 50$ for cells dissociated by the EDTA procedure, while no significant decrease in TPD$sub 50$ was observed for cells dissociated by the tryspin-EDTA procedure. (auth)

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15 p.

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Dep. NTIS

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  • Other Information: Orig. Receipt Date: 30-JUN-76

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  • Report No.: LBL--4708
  • Grant Number: W-7405-ENG-48
  • DOI: 10.2172/4148367 | External Link
  • Office of Scientific & Technical Information Report Number: 4148367
  • Archival Resource Key: ark:/67531/metadc871212

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  • November 1, 1975

Added to The UNT Digital Library

  • Sept. 16, 2016, 12:32 a.m.

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  • Oct. 12, 2017, 1:08 p.m.

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Tenforde, T.S.; Risius, J.; Beckmann, A.; Tobias, C.A. & Gurney, E. Influence of cell dissociation procedures on the tumorigenicity of Simian Virus 40 transformed fibroblasts, report, November 1, 1975; United States. (digital.library.unt.edu/ark:/67531/metadc871212/: accessed September 19, 2018), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.