[Growth of Uterine, Placental and Fetal Vasculature]. Page: 20 of 51
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18.
Ce141 while the mother was under spinal anesthesia. The spheres were
injected over a 2 minute period with the exception of the last experiment
in which they were injected over a period of 30 seconds. At the end of the
experiment the ewe was killed and the uterus with its contents removed
for tissue preparation and counting.
The uterus was then cut open, fetus removed and the uterine wall
spread out on a flat surface. The allantoic and chorionic membranes were
dissected away. Each placental cotyledon was then assigned a number and
a tracing made of the uterus and cotyledons. Each cotyledon was dissect-
ed out from the endometrium and cut into slices.
In the first eight experiments each slice was placed in a numbered
plastic test tube and the weight of .each slice determined by weighing each
test tube before and after adding the cotyledonary slice. The tubes were
then placed in a freeze dryer for 48 hours and the freeze dried tissue
crushed. Ten ml of 2N NaOH was added to the tissue paonder in each tube
.and_incubatedfor.2_hours.at 700 .C. The tubes were then centrifuged and
counted for radioactivity. Sample; of the supernatant were also counted
to ensure that the microspheres were in a pellet at the bottom of the tube.
Less than 1% of the radioactivity in the tubes was found in the supernatant.
A major disadvantage of this method is that the plastic tubes tend to warp
during the incubation and making it impractical to use an automatic count-
ing device. Because of this disadvantage the tissue from the last four
experiments was treated differently. In these studies the cotyledons were
carefully cut into slices less than 1 cm thick and placed flat on.the bottom
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Makowski, E. L. [Growth of Uterine, Placental and Fetal Vasculature]., report, October 31, 1971; Denver, Colorado. (https://digital.library.unt.edu/ark:/67531/metadc871151/m1/20/: accessed April 24, 2024), University of North Texas Libraries, UNT Digital Library, https://digital.library.unt.edu; crediting UNT Libraries Government Documents Department.