Purification and Analysis of the Mycobacteriophage Moses

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Poster presentation for the 2011 University Scholars Day at the University of North Texas discussing research on the purification and analysis of the mycobacteriophage Moses.

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1 p.

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Schade, Amy E.; Simon, Stephanie E.; Hughes, Lee E. & Benjamin, Robert C. April 14, 2011.

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Poster presentation for the 2011 University Scholars Day at the University of North Texas discussing research on the purification and analysis of the mycobacteriophage Moses.

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1 p.

Notes

Abstract: The purpose of this research is to expand the base of information related to mycobacteriophages. Mycobacteriophages can be found anywhere that there is a high presence of bacteria, especially in the top level of soil. One soil sample was collected in Denton, Texas, and an enrichment process was completed to help isolate a mycobacteriophage. A spot test was completed to ensure that a phage existed within the solution. After four steps of purification, a single phage was identified as present. The authors then prepared a high titer lysate. The sample was prepared for DNA analysis and a grid was stained to be used for electron microscopy. A restriction digest was then performed to provide insight about what type of nucleotide sequences were present in the sample. The DNA concentration was of a high enough quantity, yet it was a bit lower than the average amount of DNA present in other comparable cases. This is probably due to a smaller genome than many others, or because some of the DNA was not of good quality. Moses has a hexagonal head, which is a common feature of many mycobacteriophages. Through the careful studying of the behavior and DNA sequence of different mycobacteriophages, researchers may be able to come to conclusions that will aid them in developing cures for tuberculosis and leprosy.

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  • Eighth Annual University Scholars Day, 2011, Denton, Texas, United States

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UNT Scholarly Works

Materials from the UNT community's research, creative, and scholarly activities and UNT's Open Access Repository. Access to some items in this collection may be restricted.

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  • April 14, 2011

Added to The UNT Digital Library

  • June 18, 2012, 9:19 a.m.

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  • Nov. 21, 2017, 9:08 p.m.

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Schade, Amy E.; Simon, Stephanie E.; Hughes, Lee E. & Benjamin, Robert C. Purification and Analysis of the Mycobacteriophage Moses, poster, April 14, 2011; (digital.library.unt.edu/ark:/67531/metadc86740/: accessed May 21, 2018), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Honors College.