Damage to the DNA of microorganisms from decay of incorporated $sup 125$I and the relationship of DNA damage to lethal effects

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Iodine-125 decays by electron capture and is known to cause severe molecular damage to small organic molecules via vacancy cascades. In an examination of the biological effects of this decay mode we have labelled coliphages T1 and T4, as well as E. coli, with $sup 125$I-5-iododeoxyuridine, which is incorporated into DNA in place of thymidine. Labelled organisms are generally stored in liquid nitrogen at -196$sup 0$C during decay and are periodically assayed for loss of viability and for breakage of DNA, using neutral and alkaline sucrose gradient sedimentation techniques. Briefly, our experiments have demonstrated drastic damage to DNA from the ... continued below

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21 p.

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Krisch, R.E.; Krasin, F. & Sauri, C.J. January 1, 1975.

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Iodine-125 decays by electron capture and is known to cause severe molecular damage to small organic molecules via vacancy cascades. In an examination of the biological effects of this decay mode we have labelled coliphages T1 and T4, as well as E. coli, with $sup 125$I-5-iododeoxyuridine, which is incorporated into DNA in place of thymidine. Labelled organisms are generally stored in liquid nitrogen at -196$sup 0$C during decay and are periodically assayed for loss of viability and for breakage of DNA, using neutral and alkaline sucrose gradient sedimentation techniques. Briefly, our experiments have demonstrated drastic damage to DNA from the decay of incorporated $sup 125$I, as would be predicted from the data for small molecules. (auth)

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21 p.

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  • International conference on molecular and microdistribution of radioisotopes and biological consequences, Julich, F.R. Germany, 2 Oct 1975

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  • Report No.: CONF-751034--1
  • Grant Number: W-31-109-ENG-38
  • Office of Scientific & Technical Information Report Number: 4164027
  • Archival Resource Key: ark:/67531/metadc864865

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Office of Scientific & Technical Information Technical Reports

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  • January 1, 1975

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  • Sept. 16, 2016, 12:32 a.m.

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  • Oct. 12, 2017, 4:37 p.m.

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Krisch, R.E.; Krasin, F. & Sauri, C.J. Damage to the DNA of microorganisms from decay of incorporated $sup 125$I and the relationship of DNA damage to lethal effects, article, January 1, 1975; Illinois. (digital.library.unt.edu/ark:/67531/metadc864865/: accessed November 19, 2017), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.