Measuring the Activity of the SERCA Using Thapsigargin as a Ca+ Ion Pump Inhibitor

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Poster presentation for the 2010 University Scholars Day at the University of North Texas discussing research on measuring the activity of the SERCA using Thapsigargin as a Ca+ ion pump inhibitor.

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1 p.

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Osinde, Eliphas & Dzialowski, Edward M. (Edward Michael) April 15, 2010.

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  • Main Title: Measuring the Activity of the SERCA Using Thapsigargin as a Ca+ Ion Pump Inhibitor
  • Series Title: University Scholars Day

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Description

Poster presentation for the 2010 University Scholars Day at the University of North Texas discussing research on measuring the activity of the SERCA using Thapsigargin as a Ca+ ion pump inhibitor.

Physical Description

1 p.

Notes

Abstract: Estivation is a means through which organisms can slow metabolism to increase chances of survival in adverse conditions such as elevated temperature, and food and water shortages. The activity of the sarcoplasmic reticulum is complex and not completely understood (Wray and Burdya 2010). An enzyme within the sarcoplasmic reticulum, known as Sarcoendoplasmic Reticulum Calcium-ATPase (SERCA) is thought to play a role in regulation of metabolism and in inducing estivation. Chicks are ectotherms upon hatching. The point at which chicks transition from ectothermic regulation to enthermic regulation unclear. However by ~48 chicks are completely endothermic. During the 0-48 hour period the chick thermoregulates using shivering thermogenesis. Shivering is caused by the activation of the Sarcoendoplasmic Reticulum Calcium ATPase (SERCA) pump in the muscle cell. For every 2 Ca+ released ATP is broken down into ADP + P. By assaying the phosphate ions produced by SERCA, we can quantify SERCA. We chose the domestic chicken (Gallus gallus) as a model precocious chick. We will assay samples from the thigh and wing muscles in parallel tubes, with one containing the Ca+ ion pump inhibitor Thapsigargin as a negative control. Malachite green will be used as an indicator of phosphate ions. Samples will be analyzed by spectrophotometry at wavelengths of 620 and 655 nm.

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  • Seventh Annual University Scholars Day, 2010, Denton, Texas, United States

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UNT Scholarly Works

Materials from the UNT community's research, creative, and scholarly activities and UNT's Open Access Repository. Access to some items in this collection may be restricted.

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  • April 15, 2010

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  • June 8, 2012, 10:10 a.m.

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  • June 12, 2015, 3:48 p.m.

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Osinde, Eliphas & Dzialowski, Edward M. (Edward Michael). Measuring the Activity of the SERCA Using Thapsigargin as a Ca+ Ion Pump Inhibitor, poster, April 15, 2010; (digital.library.unt.edu/ark:/67531/metadc86141/: accessed October 20, 2017), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Honors College.