Measuring the Activity of the SERCA Using Thapsigargin as a Ca+ Ion Pump Inhibitor

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Poster presentation for the 2010 University Scholars Day at the University of North Texas discussing research on measuring the activity of the SERCA using Thapsigargin as a Ca+ ion pump inhibitor.

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Osinde, Eliphas & Dzialowski, Edward M. (Edward Michael) April 15, 2010.

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This poster is part of the collection entitled: UNT Undergraduate Student Works and was provided by the UNT Honors College to the UNT Digital Library, a digital repository hosted by the UNT Libraries. It has been viewed 773 times. More information about this poster can be viewed below.

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  • Main Title: Measuring the Activity of the SERCA Using Thapsigargin as a Ca+ Ion Pump Inhibitor
  • Series Title: University Scholars Day

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Description

Poster presentation for the 2010 University Scholars Day at the University of North Texas discussing research on measuring the activity of the SERCA using Thapsigargin as a Ca+ ion pump inhibitor.

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1 p.

Notes

Abstract: Estivation is a means through which organisms can slow metabolism to increase chances of survival in adverse conditions such as elevated temperature, and food and water shortages. The activity of the sarcoplasmic reticulum is complex and not completely understood (Wray and Burdya 2010). An enzyme within the sarcoplasmic reticulum, known as Sarcoendoplasmic Reticulum Calcium-ATPase (SERCA) is thought to play a role in regulation of metabolism and in inducing estivation. Chicks are ectotherms upon hatching. The point at which chicks transition from ectothermic regulation to enthermic regulation unclear. However by ~48 chicks are completely endothermic. During the 0-48 hour period the chick thermoregulates using shivering thermogenesis. Shivering is caused by the activation of the Sarcoendoplasmic Reticulum Calcium ATPase (SERCA) pump in the muscle cell. For every 2 Ca+ released ATP is broken down into ADP + P. By assaying the phosphate ions produced by SERCA, we can quantify SERCA. We chose the domestic chicken (Gallus gallus) as a model precocious chick. We will assay samples from the thigh and wing muscles in parallel tubes, with one containing the Ca+ ion pump inhibitor Thapsigargin as a negative control. Malachite green will be used as an indicator of phosphate ions. Samples will be analyzed by spectrophotometry at wavelengths of 620 and 655 nm.

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  • Seventh Annual University Scholars Day, 2010, Denton, Texas, United States

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UNT Undergraduate Student Works

This collection presents scholarly and artistic content created by undergraduate students. All materials have been previously accepted by a professional organization or approved by a faculty mentor. Most classroom assignments are not eligible for inclusion. The collection includes, but is not limited to Honors College theses, thesis supplemental files, professional presentations, articles, and posters. Some items in this collection are restricted to use by the UNT community.

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  • April 15, 2010

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  • June 8, 2012, 10:10 a.m.

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  • March 11, 2020, 9:28 a.m.

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Osinde, Eliphas & Dzialowski, Edward M. (Edward Michael). Measuring the Activity of the SERCA Using Thapsigargin as a Ca+ Ion Pump Inhibitor, poster, April 15, 2010; (https://digital.library.unt.edu/ark:/67531/metadc86141/: accessed March 21, 2025), University of North Texas Libraries, UNT Digital Library, https://digital.library.unt.edu; crediting UNT Honors College.

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