High-Resolution Spore Coat Architecture and Assembly of Bacillus Spores

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Elucidating the molecular architecture of bacterial and cellular surfaces and its structural dynamics is essential to understanding mechanisms of pathogenesis, immune response, physicochemical interactions, environmental resistance, and provide the means for identifying spore formulation and processing attributes. I will discuss the application of in vitro atomic force microscopy (AFM) for studies of high-resolution coat architecture and assembly of several Bacillus spore species. We have demonstrated that bacterial spore coat structures are phylogenetically and growth medium determined. We have proposed that strikingly different species-dependent coat structures of bacterial spore species are a consequence of sporulation media-dependent nucleation and crystallization mechanisms that ... continued below

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Malkin, A J; Elhadj, S & Plomp, M March 14, 2011.

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Description

Elucidating the molecular architecture of bacterial and cellular surfaces and its structural dynamics is essential to understanding mechanisms of pathogenesis, immune response, physicochemical interactions, environmental resistance, and provide the means for identifying spore formulation and processing attributes. I will discuss the application of in vitro atomic force microscopy (AFM) for studies of high-resolution coat architecture and assembly of several Bacillus spore species. We have demonstrated that bacterial spore coat structures are phylogenetically and growth medium determined. We have proposed that strikingly different species-dependent coat structures of bacterial spore species are a consequence of sporulation media-dependent nucleation and crystallization mechanisms that regulate the assembly of the outer spore coat. Spore coat layers were found to exhibit screw dislocations and two-dimensional nuclei typically observed on inorganic and macromolecular crystals. This presents the first case of non-mineral crystal growth patterns being revealed for a biological organism, which provides an unexpected example of nature exploiting fundamental materials science mechanisms for the morphogenetic control of biological ultrastructures. We have discovered and validated, distinctive formulation-specific high-resolution structural spore coat and dimensional signatures of B. anthracis spores (Sterne strain) grown in different formulation condition. We further demonstrated that measurement of the dimensional characteristics of B. anthracis spores provides formulation classification and sample matching with high sensitivity and specificity. I will present data on the development of an AFM-based immunolabeling technique for the proteomic mapping of macromolecular structures on the B. anthracis surfaces. These studies demonstrate that AFM can probe microbial surface architecture, environmental dynamics and the life cycle of bacterial and cellular systems at near-molecular resolution under physiological conditions.

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PDF-file: 3 pages; size: 0.8 Mbytes

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  • Presented at: The International Conference on Bacillus anthracis, B. cereus & B. thuringiensis, Bruges, Belgium, Aug 07 - Aug 11, 2011

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  • Report No.: LLNL-PROC-473873
  • Grant Number: W-7405-ENG-48
  • Office of Scientific & Technical Information Report Number: 1022924
  • Archival Resource Key: ark:/67531/metadc846859

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  • March 14, 2011

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  • May 19, 2016, 3:16 p.m.

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  • Nov. 29, 2016, 12:41 p.m.

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Malkin, A J; Elhadj, S & Plomp, M. High-Resolution Spore Coat Architecture and Assembly of Bacillus Spores, article, March 14, 2011; Livermore, California. (digital.library.unt.edu/ark:/67531/metadc846859/: accessed September 23, 2017), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.