Toxicology Studies on Lewisite and Sulfur Mustard Agents: Mutagenicity of Lewisite in the Salmonella Histidine Reversion Assay Final Report

Thumbnail image of item number 1 in: 'Toxicology Studies on Lewisite and Sulfur Mustard Agents: Mutagenicity of Lewisite in the Salmonella Histidine Reversion Assay Final Report'.
Thumbnail image of item number 2 in: 'Toxicology Studies on Lewisite and Sulfur Mustard Agents: Mutagenicity of Lewisite in the Salmonella Histidine Reversion Assay Final Report'.
Thumbnail image of item number 3 in: 'Toxicology Studies on Lewisite and Sulfur Mustard Agents: Mutagenicity of Lewisite in the Salmonella Histidine Reversion Assay Final Report'.
Thumbnail image of item number 4 in: 'Toxicology Studies on Lewisite and Sulfur Mustard Agents: Mutagenicity of Lewisite in the Salmonella Histidine Reversion Assay Final Report'.
Showing 1-4 of 39 pages in this report.

PDF Version Also Available for Download.

Description

The mutagenic potential of lewisite was evaluated in the standard plate incorporation method and by the preincubation modification of the Ames Salmonella/microsomal assay with tester strains TA97, TA98, TAlOO and TA102. All strains were tested with activation (20 and 50 {micro}l/ plate) and without activation. The lewisite was screened initially for toxicity with TA98 over a range of concentrations from 0.01 to 250 {micro}g of material per plate. However, concentrations selected for mutagenicity testing were adjusted to a range of 0.001 to 5 {micro}g/plate because of the sensitivity of tester strain TA102, which exhibited cytotoxicity at 0.01 ug/plate. No mutagenic ... continued below

Creation Information

Stewart, D. L.; Sass, E. J.; Fritz, L. K. & Sasser, L. B. July 31, 1989.

Context

This report is part of the collection entitled: Office of Scientific & Technical Information Technical Reports and was provided by UNT Libraries Government Documents Department to Digital Library, a digital repository hosted by the UNT Libraries. More information about this report can be viewed below.

Who

People and organizations associated with either the creation of this report or its content.

Authors

Sponsor

Publisher

Provided By

UNT Libraries Government Documents Department

Serving as both a federal and a state depository library, the UNT Libraries Government Documents Department maintains millions of items in a variety of formats. The department is a member of the FDLP Content Partnerships Program and an Affiliated Archive of the National Archives.

About | Browse this Partner

Contact Us

Corrections Questions

What

Descriptive information to help identify this report. Follow the links below to find similar items on the Digital Library.

Description

The mutagenic potential of lewisite was evaluated in the standard plate incorporation method and by the preincubation modification of the Ames Salmonella/microsomal assay with tester strains TA97, TA98, TAlOO and TA102. All strains were tested with activation (20 and 50 {micro}l/ plate) and without activation. The lewisite was screened initially for toxicity with TA98 over a range of concentrations from 0.01 to 250 {micro}g of material per plate. However, concentrations selected for mutagenicity testing were adjusted to a range of 0.001 to 5 {micro}g/plate because of the sensitivity of tester strain TA102, which exhibited cytotoxicity at 0.01 ug/plate. No mutagenic response was exhibited by any of the strains in either method used. All other tester strains showed evidence of cytoxicity (reduction in mutagen response or sparse background lawn) at 5.0 {micro}g/plate or lower.

Language

Item Type

Identifier

Unique identifying numbers for this report in the Digital Library or other systems.

  • Report No.: PNL-6872
  • Grant Number: DE-ACO6-76RL01830
  • DOI: 10.2172/1086508 | External Link
  • Office of Scientific & Technical Information Report Number: 1086508
  • Archival Resource Key: ark:/67531/metadc846047

Collections

This report is part of the following collection of related materials.

Office of Scientific & Technical Information Technical Reports

Reports, articles and other documents harvested from the Office of Scientific and Technical Information.

Office of Scientific and Technical Information (OSTI) is the Department of Energy (DOE) office that collects, preserves, and disseminates DOE-sponsored research and development (R&D) results that are the outcomes of R&D projects or other funded activities at DOE labs and facilities nationwide and grantees at universities and other institutions.

About | Browse this Collection

What responsibilities do I have when using this report?

Digital Files

When

Dates and time periods associated with this report.

Creation Date

  • July 31, 1989

Added to The UNT Digital Library

  • May 19, 2016, 9:45 a.m.

Description Last Updated

  • Dec. 2, 2016, 3:52 p.m.

Usage Statistics

When was this report last used?

Yesterday: 0
Past 30 days: 0
Total Uses: 2
More Statistics

Interact With This Report

Here are some suggestions for what to do next.

Start Reading

Thumbnail image of item number 1 in: 'Toxicology Studies on Lewisite and Sulfur Mustard Agents: Mutagenicity of Lewisite in the Salmonella Histidine Reversion Assay Final Report'.
Thumbnail image of item number 2 in: 'Toxicology Studies on Lewisite and Sulfur Mustard Agents: Mutagenicity of Lewisite in the Salmonella Histidine Reversion Assay Final Report'.
Thumbnail image of item number 3 in: 'Toxicology Studies on Lewisite and Sulfur Mustard Agents: Mutagenicity of Lewisite in the Salmonella Histidine Reversion Assay Final Report'.
Thumbnail image of item number 4 in: 'Toxicology Studies on Lewisite and Sulfur Mustard Agents: Mutagenicity of Lewisite in the Salmonella Histidine Reversion Assay Final Report'.

PDF Version Also Available for Download.

Citations, Rights, Re-Use

Stewart, D. L.; Sass, E. J.; Fritz, L. K. & Sasser, L. B. Toxicology Studies on Lewisite and Sulfur Mustard Agents: Mutagenicity of Lewisite in the Salmonella Histidine Reversion Assay Final Report, report, July 31, 1989; Richland, Washington. (digital.library.unt.edu/ark:/67531/metadc846047/: accessed April 20, 2018), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.