Isolation, Purification and Analysis of Mycobacteriophage Timshel

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This paper discusses research on mycobacteriophage (phage) timshel.

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18 p.

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Harvey, Erin; Hughes, Lee E.; Benjamin, Robert C. & Simon, Stephanie E. April 14, 2011.

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This paper is part of the collection entitled: UNT Scholarly Works and was provided by UNT Honors College to Digital Library, a digital repository hosted by the UNT Libraries. It has been viewed 1201 times , with 12 in the last month . More information about this paper can be viewed below.

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  • Main Title: Isolation, Purification and Analysis of Mycobacteriophage Timshel
  • Series Title: University Scholars Day

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Description

This paper discusses research on mycobacteriophage (phage) timshel.

Physical Description

18 p.

Notes

Abstract: The purpose of this research is to isolate, purify and sequence a unique phage in order to increase the knowledge of phage. The study began by isolating a unique phage from a soil sample. The phage was purified by a series of plaque picking and plating. Once a pure phage was obtained, DNA was extracted from a high titer lysate and a restriction fragment analysis was performed to verify the uniqueness of the phage, provide a preliminary estimate of genome size, and assess of phage family. Timshel has been classified as an A4 phage based upon the electron microscopy, gel electrophoresis, and plaque morphology. This classification has recently been verified by preliminary sequence homology analyses. The sequence also reveals the phage chromosome to be linear, 53.49 kb pairs in length, to have a 10 bp sticky end, and to encode approximately 80 predicted genes.

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  • Eighth Annual University Scholars Day, 2011, Denton, Texas, United States

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  • April 14, 2011

Added to The UNT Digital Library

  • May 18, 2012, 10:45 a.m.

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  • Nov. 21, 2017, 8:11 p.m.

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Harvey, Erin; Hughes, Lee E.; Benjamin, Robert C. & Simon, Stephanie E. Isolation, Purification and Analysis of Mycobacteriophage Timshel, paper, April 14, 2011; (digital.library.unt.edu/ark:/67531/metadc84369/: accessed December 11, 2017), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Honors College.