Purification and Analysis of Mycobacteriophage Alice

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This paper discusses research on the purification and analysis of mycobacteriophage Alice. The purpose of this research is to expand the knowledge of mycobacteriophage and analyze a single mycobacteriophage genome to be archived for future use.

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14 p.

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Manley, Coreen M.; Simon, Stephanie E.; Benjamin, Robert C. & Hughes, Lee E. April 15, 2010.

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This paper discusses research on the purification and analysis of mycobacteriophage Alice. The purpose of this research is to expand the knowledge of mycobacteriophage and analyze a single mycobacteriophage genome to be archived for future use.

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14 p.

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Abstract: The purpose of this research is to expand the knowledge of mycobacteriophage and analyze a single mycobacteriophage genome to be archived for future use. Mycobacteriophages are found in soil almost anywhere with an abundance of host mycobacteria. The analysis of mycobacteriophages may help lead to breakthroughs in DNA recombination and help the scientific community in the search for a cure for genetic diseases as well as diseases caused by mycobacteria including tuberculosis. In this study, a single phage, Alice, was isolated from a soil sample found in a compost pile. The sample was directly plated using M. smegmatis as the host organism. After the plaques were verified to be formed by phage, a series of dilutions to isolate and purify the phage were performed. The final product from the dilutions was a high titer lysate that, when prepared, was used to extract the DNA from the phage which was then sequenced and analyzed. Compared to other organisms, mycobacteriophage are relatively unknown. Only about 60 have been sequenced, so the range of comparison for conservation in Alice's genome is very slim. In this study the authors hope to increase the scientific community's knowledge of mycobacteriophages and how they affect the areas in which they are found.

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  • Seventh Annual University Scholars Day, 2010, Denton, Texas, United States

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UNT Scholarly Works

Materials from the UNT community's research, creative, and scholarly activities and UNT's Open Access Repository. Access to some items in this collection may be restricted.

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Purification and Analysis of Mycobacteriophage Alice (Presentation)

Purification and Analysis of Mycobacteriophage Alice

Presentation for the 2010 University Scholars Day at the University of North Texas discussing the purification and analysis of mycobacteriophage Alice.

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Purification and Analysis of Mycobacteriophage Alice, ark:/67531/metadc93246

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  • April 15, 2010

Added to The UNT Digital Library

  • May 18, 2012, 10:45 a.m.

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  • Nov. 21, 2017, 9:08 p.m.

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Manley, Coreen M.; Simon, Stephanie E.; Benjamin, Robert C. & Hughes, Lee E. Purification and Analysis of Mycobacteriophage Alice, paper, April 15, 2010; (digital.library.unt.edu/ark:/67531/metadc84343/: accessed October 18, 2018), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Honors College.