Methods for Engineering Sulfate Reducing Bacteria of the Genus Desulfovibrio

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Sulfate reducing bacteria are physiologically important given their nearly ubiquitous presence and have important applications in the areas of bioremediation and bioenergy. This chapter provides details on the steps used for homologous-recombination mediated chromosomal manipulation of Desulfovibrio vulgaris Hildenborough, a well-studied sulfate reducer. More specifically, we focus on the implementation of a 'parts' based approach for suicide vector assembly, important aspects of anaerobic culturing, choices for antibiotic selection, electroporation-based DNA transformation, as well as tools for screening and verifying genetically modified constructs. These methods, which in principle may be extended to other sulfate-reducing bacteria, are applicable for functional genomics investigations, ... continued below

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Chhabra, Swapnil R; Keller, Kimberly L. & Wall, Judy D. March 15, 2011.

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Sulfate reducing bacteria are physiologically important given their nearly ubiquitous presence and have important applications in the areas of bioremediation and bioenergy. This chapter provides details on the steps used for homologous-recombination mediated chromosomal manipulation of Desulfovibrio vulgaris Hildenborough, a well-studied sulfate reducer. More specifically, we focus on the implementation of a 'parts' based approach for suicide vector assembly, important aspects of anaerobic culturing, choices for antibiotic selection, electroporation-based DNA transformation, as well as tools for screening and verifying genetically modified constructs. These methods, which in principle may be extended to other sulfate-reducing bacteria, are applicable for functional genomics investigations, as well as metabolic engineering manipulations.

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  • Report No.: LBNL-4535E
  • Grant Number: DE-AC02-05CH11231
  • Office of Scientific & Technical Information Report Number: 1015996
  • Archival Resource Key: ark:/67531/metadc841174

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Office of Scientific & Technical Information Technical Reports

Reports, articles and other documents harvested from the Office of Scientific and Technical Information.

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  • March 15, 2011

Added to The UNT Digital Library

  • May 19, 2016, 3:16 p.m.

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  • June 16, 2016, 12:48 p.m.

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Chhabra, Swapnil R; Keller, Kimberly L. & Wall, Judy D. Methods for Engineering Sulfate Reducing Bacteria of the Genus Desulfovibrio, book, March 15, 2011; Berkeley, California. (digital.library.unt.edu/ark:/67531/metadc841174/: accessed June 25, 2018), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.